IntroductionMesenchymal stem cells (MSCs) are promising candidates for cell-based therapies. Human platelet lysate represents an efficient alternative to fetal bovine serum for clinical-scale expansion of MSCs. Different media used in culture processes should maintain the biological characteristics of MSCs during multiple passages. However, bone marrow-derived MSCs and adipose tissue-derived MSCs have not yet been directly compared with each other under human platelet lysate conditions. This study aims to conduct a direct head-to-head comparison of the biological characteristics of the two types of MSCs under human platelet lysate-supplemented culture conditions for their ability to be used in regenerative medicine applications.MethodsThe bone marrow- and adipose tissue-derived MSCs were cultured under human platelet lysate conditions and their biological characteristics evaluated for cell therapy (morphology, immunophenotype, colony-forming unit-fibroblast efficiency, proliferation capacity, potential for mesodermal differentiation, secreted proteins, and immunomodulatory effects).ResultsUnder human platelet lysate-supplemented culture conditions, bone marrow- and adipose tissue-derived MSCs exhibited similar fibroblast-like morphology and expression patterns of surface markers. Adipose tissue-derived MSCs had greater proliferative potential than bone marrow-derived MSCs, while no significantly difference in colony efficiency were observed between the two types of cells. However, bone marrow-derived MSCs possessed higher capacity toward osteogenic and chondrogenic differentiation compared with adipose tissue-derived MSCs, while similar adipogenic differentiation potential wase observed between the two types of cells. There were some differences between bone marrow- and adipose tissue-derived MSCs for several secreted proteins, such as cytokine (interferon-γ), growth factors (basic fibroblast growth factor, hepatocyte growth factor, and insulin-like growth factor-1), and chemokine (stem cell-derived factor-1). Adipose tissue-derived MSCs had more potent immunomodulatory effects than bone marrow-derived MSCs.ConclusionsAdipose tissue-derived MSCs have biological advantages in the proliferative capacity, secreted proteins (basic fibroblast growth factor, interferon-γ, and insulin-like growth factor-1), and immunomodulatory effects, but bone marrow-derived MSCs have advantages in osteogenic and chondrogenic differentiation potential and secreted proteins (stem cell-derived factor-1 and hepatocyte growth factor); these biological advantages should be considered systematically when choosing the MSC source for specific clinical application.

Under unloading condition, the characteristics of rock are different from them under loading condition. This paper talks about the characteristics of Ordovician limestone under loading and unloading condition of high compress which were taken from North Tian Mountain. The testing results indicated that: The lateral deformation and bulk deformation of rock sample are clearly in the process of unloading. Plastic deformation is not notability in the unloading condition. After the peak value in the stress-strain curve, the stress decrease quickly, it is showed that the brittleness characteristic is the better remarkable under unloading condition than loading condition. Under unloading condition, deformation modulus E50 and Possion ratio μ is quadric with the confining compress.

Fixing Diesel Particulate Filter (DPF) in diesel is an effective measure to solve the particulate emissions. As PM is accumulated in DPF constantly, it will increase diesel exhaust resistance and affect diesel performance. Through building the exhaust resistance test rig and diesel test bench, the thesis studies the effects of particulate matter loading in DPF on diesel exhaust resistance and diesel performance. The results show that it is the most effective to conduct silicon carbide filter’s thermal regeneration when particulate matter loading is 8g/L.

The Strength characteristic of rock are different from them under unloading condition. The Strength characteristic of Ordovician limestone under loading and unloading condition of high compress which were taken from North Tian Mountain. It indicated that : Under unloading condition cohesion c and inner friction angle decrease according to loading condition, and the residual strength has more decrease than peak strength. Under unloading, peak compress strength and residual compress strength decrease distinctly, and the decrease reduces along with confining compress increasing.

The one-step synthesis of methyl isobutyl ketone from acetone (MIBK) and H2 in liquid phase was studied over Ni-zirconia catalyst prepared by precipitation. The performances of Ni/ZrO2 catalysts were evaluated by the conversion of acetone and the selectivity of MIBK. The effect of different preparation conditions such as aging temperature, calcination temperature, nickel content and reduction temperature was investigated. The results showed that high acetone conversions of up to 33% and MIBK selectivities of up to 85% were recorded at a nickel content of 13.0 wt % and the reduction temperature of 400°C.