Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a key enzyme involved in CO 2 assimilation during photosynthesis. Rubisco activation depends on the activity of Rubisco activase (RCA). We performed 3 0 /5 0 rapid amplification of cDNA ends (RACE) and reverse transcription polymerase chain reaction (RT-PCR) to amplify the 3 0 and 5 0 end sequences of RCA genes from hickory. We obtained two full-length gene sequences, designated CcRCAa and CcRCAb. The two corresponding cDNAs are divergent in both the translated and 3 0 untranslated regions. The analysis of the genomic DNA sequences suggested that the corresponding mRNAs are transcripts of two different genes and not the products of a single alternatively spliced pre-mRNA. We examined the expression of CcRCAa and CcRCAb in hickory leaves at various stages of development by quantitative real-time PCR (qRT-PCR) analysis. The results suggest that RCA genes play an important role in development and environmental responses. These results provide a basis for modulating RCA gene expression to improve the photosynthetic rate and plant growth in hickory.
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