Background Venous thromboembolism (VTE) is a life-threatening cardiovascular syndrome that characterized by the imbalance of hemostasis and thrombosis and the formation of thrombi in the blood vessels. The aim of this study was to elucidate the clinical impact of the PAI-1 4G/5G polymorphism in Chinese patients with VTE. Methods A total of 169 subjects (89 VTE, 10 hyperbilirubinemia, 10 hyperlipidemia and 60 healthy controls) were recruited at Peking Union Medical College Hospital. The accuracy of the TaqMan-MGB RT-PCR method for detecting F5 G1691A (FVL) and PAI-1 4G/5G polymorphisms was evaluated by using sequencing method as the gold standard. Besides, the association of the PAI-1 4G/5G polymorphism with susceptibility, treatment efficacy and recurrence status of VTE in Chinese population were explored. Eventually, the plasma PAI-1 antigen levels and PAI-1 4G/5G polymorphisms were determined on additional 64 subjects (32 VTE and 32 healthy controls) simultaneously. Results The TaqMan-MGB RT-PCR method was proven to be highly accurate in determining the FVL and PAI-1 4G/5G polymorphisms without interference from bilirubin and lipids in the samples. No obvious correlation of the PAI-1 4G/5G polymorphism with VTE was observed in our study by using five genetic models (allele, genotype, dominant, recessive and additive). Additionally, we also observed that individuals with the 4G/5G genotype had lower neutrophil counts and neutrophil-to-lymphocyte ratio (NLR) than the 5G/5G genotype. Furthermore, we found that the patients with the 5G/5G genotype were more likely to achieve complete recanalization compared to the 4G/4G genotype. In addition, individuals carrying the 5G/5G genotype were more likely to develop a recurrence-free status as compared to individuals with the 4G/4G or 4G/5G genotypes. PAI-1 antigen levels in the VTE group were significantly higher than those in the HC group. However, there was no significant difference in the antigen levels of PAI-1 among subjects carrying various genotypes in the VTE group or HC group. Conclusion The PAI-1 4G/5G polymorphism has potential value in assessing the prognosis of Chinese patients with VTE. Our study has laid the foundation for the application of PAI-1 4G/5G polymorphism in the personalized management and monitoring of patients with VTE.

IntroductionThe delta check quality control measure has been applied in haematology testing for a long time. Although the delta check graph of haematology test had been reported by Berend Houwen in 1989, problems still exist in its clinical application. This study aims to provide a simplified and practical process for the improvement of validity in haematology testing.MethodsWe analysed a total of 5260 patients with two sequential complete blood counting results, taken in 1 week, to verify the delta check curve. The delta check equations were simulated using the sigma plot 13.0 software. Validations of test results were performed on patients from the haematology, oncology and other departments.ResultsThree equations of the delta check limits for mean corpuscular volume (MCV), haemoglobin, white blood cell and platelets were simulated. The following two limitation lines were selected: limit A (ΔA) and limit B (ΔB), wherein ΔA was to simplify the review rules and ΔB to check random errors. Delta check procedures including rules and equations were established for clinical use, and the re‐inspection rate was reduced in different departments using the delta check model in clinical validation.ConclusionThe simplified delta check limitation formulae are meaningful for data review and may help the laboratory technician optimize workflow and reduce workload.

Background Pituitary stalk interruption syndrome (PSIS) is a rare disease caused by congenital pituitary anatomical defects. The underlying mechanisms remain unclear, and the diagnosis is difficult. Here, integrated metabolomics and lipidomics profiling were conducted to study the pathogenesis of PSIS. Methods Twenty-one patients with PSIS (BD group) and twenty-three healthy controls (HC group) were enrolled. Basal information and seminal plasma samples were collected. Untargeted metabolomics and lipidomics analyses were performed using ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Results The metabolomics and lipidomics profiles of patients with PSIS changed. The prolactin signaling pathway and biosynthesis of amino acids were the main differentially modified metabolic pathways. The main differentially modified metabolites were triacylglycerols (TGs), phosphatidylethanolamine (PE), sphingomyelin (SM), ceramide (Cer) and phosphatidylcholines (PCs). Pregnenolones and L-saccharopine could achieve a diagnosis of PSIS. Conclusions Pregnenolones and L-saccharopine are potential biomarkers for a PSIS diagnosis.

547291872@qq.com, Key Words: Materials Studio; molecular mechanics; mechanism; calcite; fluorite Abstract:The floatability influence of sodium oleate and oleic acid on fluorite, calcite were studied by flotation test. Zeta potential of fluorite and calcite was tested by Zeta Potential Analyzer. By Materials Studio software, the reagent action on mineral surface was simulated and the surface energy changes in this process were calculated. The research results shows that, the collect capability of sodium oleate on mineral is better than oleic acid, the floatability of fluorite is better than calcite, it is because that sodium oleate and oleic acid collect mineral by chemical adsorption of oleate ions on fluorite and calcite surface, the adsorption strength of oleate ions on fluorite is stronger than water and OH -, while on calcite it is just opposite. In the flotation system, the collecting ability of oleate ions on fluorite is strong, and less affected by the water molecules and OH -, however, the collecting ability of oleate ions on calcite is weaker than water molecules and OH -ions.