ABSTRACT.-Terzariol M., Hünning P.S., Brambatti G., Albuquerque L., Neumann C. & Pigatto J.A. T. 2016 The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05% brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue ® ) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with a balanced salt solution injection and the cornea was excised too. All the corneas were evaluated by scanning electron microscopy to evaluate the changes on the endothelium caused by the brilliant blue dye. There were no significant differences between the right corneal endothelium cells and the left corneal endothelium cells with scanning electron microscopy after intracameral use of 0.05% brilliant blue dye. The 0.05% brilliant blue dye concentration did not cause deleterious effects for the swine corneal endothelium after intracameral use and can be a choice for safe staining of the anterior capsule of the lens in cataract surgery.INDEX TERMS: Eye, brilliant blue 0.05%, toxicity, corneal endothelium, swine.

RESUMOAvaliaram-se duas novas técnicas de inseminação laparoscópica intrauterina com o uso de agulha espinhal em ovinos. Foram realizadas quatro etapas experimentais, sendo os animais separados em dois grupos em cada etapa. Para tanto, foram utilizados os posicionamentos quadrupedal e dorsal. Realizou-se a inseminação de 80 ovinos após a sincronização de cio. Verificou-se que os acessos laparoscópicos propostos foram viáveis para a inseminação em ovinos. Considerou-se que a inseminação em posicionamento quadrupedal foi tecnicamente mais difícil se comparada ao acesso laparoscópico em decúbito dorsal, porém, com o aprimoramento da técnica e na dependência dos resultados quanto aos índices de prenhez, poderá se tornar procedimento adequado para a inseminação de ovelhas. Palavras-chave: ovelhas, videolaparoscopia, agulha espinhal, útero, prenhez ABSTRACT This study evaluated two new laparoscopic intrauterine insemination techniques with spinal needles in sheep.

Procurou-se comparar duas técnicas de ovariosalpingohisterectomia (OSH): vídeo-assistida com dois portais e convencional em cadelas, ambas com o uso de equipamento Ligasure atlas®. Para tanto, foram utilizados 18 animais separados em dois grupos, sendo no primeiro grupo realizada a OSH por celiotomia e no segundo pelo acesso vídeo-assistido. Os procedimentos videocirúrgicos foram realizados por meio de dois trocartes dispostos nas regiões umbilical e pré-púbica, com os cães posicionados em decúbito dorsal. Em ambos os grupos, o único método de hemostasia empregado foi o Ligasure atlas®. Não houve diferença significativa acerca do tempo operatório, das complicações trans e pós-operatórias e das perdas sanguíneas. Conclui-se que a OSH vídeo-assistida com o uso de dois portais e Ligasure atlas® e a técnica convencional com o emprego do mesmo equipamento são rápidas, seguras e efetivas em cadelas.

The goal of this study was to describe the normal values of corneal endothelial cell density and hexagonality in healthy rabbits’ eyes using contact specular microscopy. Both eyes of 18 rabbits, males and females, of different ages were evaluated. The eyes were divided into three groups of 12 each according to the age range of the animals: G1 (6 months old), G2 (12 months old), and G3 (48 months old). Parameters studied included mean endothelial cell density and hexagonality. For Group I, the mean cell density was 2307 cells per mm2. For GII, the mean cell density was 1895 cells per mm2. For G III, the mean cell density was 1818 cells per mm2. Cell density decreased significantly with aging (P<0.001) among the three groups. Pleomorphism in the rabbits of GI was 74.33±10.08. In the rabbits of GII it was 71.83±11.38. In the rabbits of GIII it was 64.02±28.80. Significant differences (P<0.001) were evident between GI and GIII and GII and GIII but not between Groups I and II. The results showed a decrease in number of cells and hexagonality with aging. Corneal endothelium of rabbits suffers changes due to advancing age both in terms of cell density and morphology.

Background: Primary pulmonary neoplasia is rare in domestic cats, with fewer than 1% of all tumors diagnosed in this species. Primary lung tumors have a high percentage of metastases, observed in up to 75% of cases in cats. The lung-digit syndrome is characterized by primary pulmonary neoplasms with metastases to the extremities of the limbs, mainly distal phalanges, possibly involving several digits and more than one limb. This report describes a case of pulmonary carcinoma metastasis in a cat digit. Case: A 16-year-old neutered female cat was showing increased volume of the fourth digit was examined at the University of Caxias do Sul veterinary clinic. The animal also showed signs of progressive weight loss, apathy and limping on the right thoracic limb. After clinical evaluation, the animal was sent for radiographic examination of the right metacarpal/ phalangeal region and the thorax. A radiopaque structure was observed with discreet central regions of radiolucency, measuring approximately 3 cm in height, 3.4 cm in length and 2.4 cm in depth in the left caudal lung, suggesting neoplasia or pulmonary abscess. Digit image revealed osteolysis of the right and middle distal phalanx measuring 2 cm in height, 3 cm in breadth and 1 cm in length. In view of these radiographic changes, we performed fine needle aspiration cytology of the digit and a nodule in the region of the skeletal musculature of the right thoracic limb. Cytological evaluation revealed cells had cilia on their surface (compatible with respiratory epithelium). The cytologic findings of the fine-needle biopsy were suggestive of carcinoma. After stabilization, the digit was amputated. The material was sent to the laboratory of the Federal University of Pelotas for histopathological examination. Histological evaluation of a digital pulmonary carcinoma metastasis was confirmed. In view of the prognosis and clinical evolution of the disease, the caretaker chose euthanasia. Necropsy and histopathological evaluation of the lung and other organs were not authorized. Discussion: According to the literature, primary lung tumors have a high percentage of metastases, observed in up to 75% of cases in felines. The size of the tumor mass is associated with its metastatic potential, and lung tumors smaller than 1 cm in diameter usually do not present metastases. In the present case, the mass in the lung was greater than 1 cm in size and the animal already presented metastasis in the digit and in the skeletal musculature. In addition to the thoracic cavity, sites where metastases from lung tumors may occur include regional lymph nodes, skin, eyes, skeletal muscle, multiple abdominal organs, and digits. Cats with pulmonary carcinoma and digital metastasis have a median survival 67 days after diagnosis, with intervals of 6 to 122 days reported. Most patients are euthanized due to persistent symptoms of lameness, lethargy and anorexia or evolution of respiratory signs, in addition to the short survival time associated with poor quality of life. In the case reported he...