BACKGROUND: School performance encompasses several forms of representation, and this has been identified as stressing factors with negative repercussions on teachers’ health. In 2020 this scenario was affected by the COVID-19 pandemic, with yet unknown consequences on teachers’ health. Our hypothesis is that the disease affected both the quality of life and work ability of teachers. OBJECTIVE: To identify the association between burnout syndrome, work ability, quality of life and physical activity in basic education teachers during the COVID-19 pandemic in the city of Campo Grande, Brazil. METHODS: Data collection took place from April 2020 to April 2021 through an online form linked to Google Forms. Quality of life was measured using the WHOQOL-bref, Work Ability through Work Ability Index (WAI) and burnout syndrome aspects through MBI-HSS. The association between variables was assessed by Pearson’s correlation test with p <  0,05. RESULTS: Teachers self-rated their quality of life positively (good/very good = 55.9%), showed good (43,1%) or very good (27,7%) work ability, and had low emotional fatigue (41.6%), low depersonalization (75.7%) and high personal fulfillment (58.4%). The highest correlations indicate that the greater the emotional fatigue, the lower the WAI (r = – 0.60); and the higher the WAI, the higher the quality of life (all p <  0,05). CONCLUSION: The associations indicate that those teachers who showed greater emotional fatigue showed less ability to work. In addition, a negative association between emotional fatigue and physical, social and environmental domain of quality of life was also expressed, which may be relevant to the work environment.