Wind-driven upwelling followed by relaxation results in cycles of cold nutrient-rich water fueling intense phytoplankton blooms followed by nutrient depletion, bloom decline, and sinking of cells. Surviving cells at depth can then be vertically transported back to the surface with upwelled waters to seed another bloom. As a result of these cycles, phytoplankton communities in upwelling regions are transported through a wide range of light and nutrient conditions. Diatoms appear to be well suited for these cycles, but their responses to them remain understudied. To investigate the bases for diatoms’ ecological success in upwelling environments, we employed laboratory simulations of a complete upwelling cycle with a common diatom, Chaetoceros decipiens, and coccolithophore, Emiliania huxleyi. We show that while both organisms exhibited physiological and transcriptomic plasticity, the diatom displayed a distinct response enabling it to rapidly shift-up growth rates and nitrate assimilation when returned to light and available nutrients following dark nutrient-deplete conditions. As observed in natural diatom communities, C. decipiens highly expresses before upwelling, or frontloads, key transcriptional and nitrate assimilation genes, coordinating its rapid response to upwelling conditions. Low-iron simulations showed that C. decipiens is capable of maintaining this response when iron is limiting to growth, whereas E. huxleyi is not. Differential expression between iron treatments further revealed specific genes used by each organism under low iron availability. Overall, these results highlight the responses of two dominant phytoplankton groups to upwelling cycles, providing insight into the mechanisms fueling diatom blooms during upwelling events. IMPORTANCE Coastal upwelling regions are among the most biologically productive ecosystems. During upwelling events, nutrient-rich water is delivered from depth resulting in intense phytoplankton blooms typically dominated by diatoms. Along with nutrients, phytoplankton may also be transported from depth to seed these blooms then return to depth as upwelling subsides creating a cycle with varied conditions. To investigate diatoms’ success in upwelling regions, we compare the responses of a common diatom and coccolithophore throughout simulated upwelling cycles under iron-replete and iron-limiting conditions. The diatom exhibited a distinct rapid response to upwelling irrespective of iron status, whereas the coccolithophore’s response was either delayed or suppressed depending on iron availability. Concurrently, the diatom highly expresses, or frontloads, nitrate assimilation genes prior to upwelling, potentially enabling this rapid response. These results provide insight into the molecular mechanisms underlying diatom blooms and ecological success in upwelling regions.

In contrast to birds, the contribution of body reserves to sustain reproductive activities of migratory bats has not being examined. We used C stable isotope analysis to track the importance of nutrients stored in body tissues of the lesser long-nosed bat ( Leptonycteris yerbabuenae ) in Central Mexico. The bat migrates seasonally between areas dominated by vegetation types with contrasting C stable isotope values: in spring–summer, it forages in cactus forests before commuting to dry and wet forests, where mating and births occur. We collected breath and whole blood from nonreproductive individuals in a cactus forest in spring–mid-summer, from mating individuals in an evergreen forest in mid-late summer, and from lactating females in winter in a tropical deciduous forest. We also collected hair and milk from lactating females and several tissues from naturally aborted fetus in late autumn. We tested the hypothesis that nutrient reserves accumulated in cactus forest contribute to the maintenance of adults when they commute to their reproductive grounds, to the construction of offspring tissues during pregnancy, and to the production of milk. The importance of energy stores accumulated in cactus forests to fuel oxidative metabolism was marginal for mating males but it was high for some mating females. Nutrient stores accumulated in cactus forests contributed to ~50% of synthesis of fetus tissues but their contribution for milk production was negligible. Female lesser long-nosed bats can be described as capital-income breeders in relation to the development of offspring during gestation and as incomer breeders in relation to lactation. En contraste con las aves, la contribución de las reservas corporales para mantener las actividades reproductivas no ha sido evaluada en los murciélagos migratorios. En este estudio, usamos análisis de isótopos estables de C para reconstruir la importancia de los nutrientes almacenados en los tejidos del murciélago magueyero menor ( Leptonycteris yerbabuenae ) en el centro de México. Este murciélago migra estacionalmente entre áreas dominadas por tipos de vegetación con valores contrastantes de isótopos estables de C: la especie forrajea en bosques de cactáceas en primavera-verano antes de moverse a bosques secos y húmedos donde ocurren el apareamiento y los nacimientos. Se colectaron muestras de aliento y sangre entera de individuos no reproductivos en un bosque de cactáceas en primavera y mediados del verano, de individuos en etapa de apareamiento en un bosque húmedo a mediados y finales del verano, y de hembras lactantes en el invierno en un bosque seco tropical. Además, se colectaron muestras de pelo y leche de las hembras lactantes, y de varios tejidos de fetos a finales del otoño. Se probó la hipótesis de que las reservas de nutrientes acumuladas en el bosque de cactáceas contribuyen al mantenimiento de los adultos cuando se mueven a sus sitios de apareamiento, a la construcción de tejidos de las crías durante la preñez, y a la producción de leche. La importancia de las reservas de energía acumuladas en los bosques de cactáceas fue marginal para los machos y fue alta para algunas hembras durante el apareamiento. Los nutrientes acumulados en los bosques de cactáceas contribuyeron en hasta el 50% de la síntesis de tejidos de los fetos pero su contribución para la producción de leche fue de poca importancia. Las hembras de los murciélagos magueyeros menores siguen una estrategia reproductiva mixta de uso de reservas acumuladas y de nutrientes externos en relación al desarrollo de las crías durante la gestación, y una estrategia de uso de nutrientes externos en relación a la lactancia.

Phytoplankton communities in upwelling regions experience a wide range of light and nutrient conditions as a result of upwelling cycles. These cycles can begin with a bloom at the surface followed by cells sinking to depth when nutrients are depleted. Cells can then be transported back to the surface with upwelled waters to seed another bloom. In spite of the physicochemical extremes associated with these cycles, diatoms consistently outcompete other phytoplankton when upwelling events occur. Here we simulated the conditions of a complete upwelling cycle with a common diatom, Chaetoceros decipiens, and coccolithophore, Emiliania huxleyi. We show that while both organisms exhibited physiological and transcriptomic plasticity, the diatom displayed a distinct response enabling it to rapidly shiftup growth and nitrate assimilation when returned to light and nutrients. As observed in natural diatom communities, C. decipiens frontloads key transcriptional and nitrate assimilation genes coordinating its rapid response. Low iron simulations showed that C. decipiens is capable of maintaining this response when iron is limiting whereas E. huxleyi could not. Differential expression between iron treatments further revealed molecular mechanisms used by each organism under low iron availability. Overall, these results highlight the responses of two dominant phytoplankton groups to upwelling cycles, providing insight into the mechanisms fueling diatom success during upwelling events in current and future oceans. Correspondence: amarchetti@unc.edu Lampe et al. | bioRχiv | May 1, 2020 | 1-11 Low Light, High Nutrients (T2, T3) High Light, High Nutrients (Shift-up; T4) High Light, Low Nutrients (Shift-Down; T6) Sinking Balanced Growth (T1, T5)