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Herein, a new triboelectric energy‐harvesting method by a direct transfer‐triboelectric nanogenerator (DT‐TENG) in a streaming flow is proposed. Previously, most of the fluid‐based‐triboelectric nanogenerators (Flu‐TENGs) generate the electricity by electrostatic induction, which is one of the indirect charge transfer methods. To enhance the triboelectric effect of liquid from the surface of dielectric material, negative dielectric surface treatment is focused. The significance of a novel direct charge transfer method to generate high output power of Flu‐TENG is first reported herein. From the experiment, the maximum power of DT‐TENG reaches up to 28.4 μW. The findings not only enhance the output power in the streaming flow but also provide a paradigm‐shifting technique to harvest mechanical energy in the flow electrification.