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Erythrocytes from rats on tocopherol-deficient and tocopherol-supplemented diets gave similar low values for lipid peroxides by the thiobarbituric acid test. Treatment of the cells from tocopherol-deficient rats with dialuric acid caused a marked increase in lipid peroxitles and this closely paralleled the degree of hernolysis. Shaking of the red cells from tocopherol-deficient arlimals in a n atmosphere of oxygen also resulted in a slow lipid peroxidatio~l which again paralleled the degree of hernolysis. Addition of a-tocopherol to a suspension of erythrocytes from tocopherol-deficient rats decreased or completely prevented (depending upon concentration) hemolysis by dialuric acid and also decreased lipid peroxitle formation.It is concluded that dialuric acid probably acts by catalyzing the formation of lipid peroxides in the unsaturated lipids of the membrane of erythrocytes from tocopherol-deficient animals. Tocopherol presumably inhibits peroxide formation, and therefore hemolysis, by virtue of its antioxidant action.

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Mammalian catalase and cytochrome oxidase are strongly inhibited by the hydroxy derivatives of phenothiazine and by p-hydroxylaminobenzenesulphonamide. Phenothiazine, sulphanilamide, and sulphapyridine have little or no effect. Cytochrome-c is irreversibly reduced by the hydroxy-sulphanilamide, as indicated by spectroscopic observation. The inhibitory activity apparently depends on the presence of the hydroxyl group. The relation of these findings to the vermicidal and bactericidal action of the compounds is discussed.Phenothiazine and thionol have no effect on Ascaris lumbricoides in vitro.