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We investigated whether the bovine herpesvirus 1.1 (BHV1) ORF, a homologue of the herpes simplex virus 1 (HSV1) UL25 gene, represented a functional gene. The BHV1 UL25 ORF, which is located at positions 60602<--62398 of the viral genome, generated a 4.5 kb transcript accumulating at low abundance as soon as 3 hours p.i. after which the levels increased up to 12h p.i. and remained constant up to 24 hours p.i. In addition, UL25 transcription initiated at 303 bases upstream from the translation initiation codon, corresponding to 26 and 354 b downstream from putative TATA and CAAT boxes, respectively, thus providing evidence that these elements function as the UL25 promoter. Western blotting of BHV1-infected cell lysates, using a BHV1-UL25 specific antiserum generated against a T7-Tag/UL25 fusion recombinant protein expressed in E. coli, detected a 63 kDa protein of the expected size (63,083 Da) whose expression profile followed that of its transcript. The synthesis of the 63 kDa protein was abrogated by a DNA synthesis inhibitor, unambiguously demonstrating that the viral specific protein expressed from the BHV1 UL25 ORF belongs to the gamma2 class.

Notes editor, announced a special Y2K issue of Notes to take us into the millennium. Scheduled for May 2000, it will offer an overview of music librarianship including collection development, technical services, reference services, sources, archives, publishing, and sound recordings. Notes remains under budget, at about 1,000 pages per volume. New type-setting software, whereby the type goes directly onto film, will be employed with the March issue and will facilitate the production of electronic copy.Susan Hitchens, Convention Manager, reported that 4 1 5 regular members (including 14 students) and 95 contributors were present at the LA meeting for a total of 5 10 attendees. The next meeting will be in Louisville, Kentucky. Ruth Ann McTyre (Baylor) is Program Chair for the Kentucky meeting.

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