Aphids and thrips are very important pest species of many greenhouse and field plants in the world. In recent years, there have been attempts to identify plants for their insecticidal property with a view to find out suitable alternatives to the indiscriminate use of chemical pesticides, which are a risk for human health and the environment. The aim of this work was to evaluate the insecticidal effect of Eucalyptus globulus Labill essential oil (EO) against Aphis nerii Boyer de Fonscolombe (Hemiptera: Aphididae) and Gynaikothrips ficorum Marchal (Thysanoptera: Ploeothridae). The EO was extracted by hydrodistillation and the bioassay was evaluated by toxicity contact method, using solutions at different concentrations and for different time intervals. The EO was more toxic to G. ficorum (LC 50 = 0.031µL cm-2) than A. nerii (LC 50 = 0.099µL cm-2) on filter discs at 12 h of exposure. These results suggested that EO from E. globulus constitute a good alternative for the control of A. nerii and G. ficorum, considering their insecticidal effects at low applied concentrations and for short periods of time.