The aim of the present study was to determine the effects of Luteolin (LUT) on semen quality, oxidative stress, apoptosis, acrosomal integrity, mitochondrial membrane potential and dead sperm ratio in rabbits. Ejaculates from six New Zealand rabbits were collected, evaluated and pooled. The pooling was divided into five groups as control (no additive) LUT 25 µM, LUT 50 µM, LUT 100 µM and LUT 200 µM and LUT added. It was then filled into a falcon tube with Tris-based extender at a final concentration of approximately 35 x 10 6 spermatozoa. Diluated rabbit semen samples were drawn into frozen and thawed. Frozen semen straws were thawed at 37°C in 30 seconds. According to our findings, no statistical difference was found between all doses of luteolin and the control group in the CASA (computer assisted sperm analysis) analysis performed at 4°C. However, total motility, progressive motility and rapid sperm percentage were found to be higher in the frozen and thawed rabbit semen at a dose of LUT 50 µM compared to the other groups (p<0.05). While amplitude of lateral head displacement (ALH) and beat cross-frequency (BCF) values were found at the lowest dose of LUT 200 µM, a statistically significant difference was observed between the other groups. When the flow cytometry results were examined, no statistical difference was found between the rate of dead sperm, acrosomal integrity, mitochondrial membrane potential and apoptosis rate. Morever, the H 2 O 2 percentage was found to be lower in all experimental groups compared to the control group (p<0.001). In conclusion, the addition of LUT in long-term storage of rabbit semen provided a protective effect for spermatozoa with its antioxidative properties against damage caused by cryopreservation.

Bu çalışmanın amacı, tiazolidin'in (Tiazolidin-4-karboksilik asit (4S)-2-(4hidroksi-3-metoksifenil) zebra balığı (Danio rerio) solungaç ve karaciğer dokusunda asetilkolinesteraz enzim (AChE) aktivitesi ve total protein (TP) düzeyleri üzerindeki etkilerinin araştırılmasıdır. Zebra balıkları tiazolidin'in 0,2 ppm, 0,4 ppm ve 0,6 ppm farklı dozlarına 96 saat süreyle maruz bırakılmıştır. AChE enzim aktivitesi karaciğer dokusunda, tiazolidin'in 0,2 ppm, 0,4 ppm ve 0,6 ppm doz gruplarında, kontrol grubuna oranla artmıştır. Solungaç dokusunda ise, tiazolidin'in 0,2 ppm, 0,4 ppm ve 0,6 ppm doz gruplarında, kontrol grubuna oranla AChE enzim aktivitesinin azaldığı görülmüştür. Total protein seviyesi karaciğer dokusunda, tiazolidin'in 0,2 ppm, 0,4 ppm ve 0,6 ppm doz gruplarında, kontrol grubuna oranla azalmıştır. Solungaç dokusunda ise, tiazolidin'in 0,2 ppm, 0,4 ppm ve 0,6 ppm doz gruplarında, kontrol grubuna oranla total protein seviyelerinin önemli sayılabilecek oranda arttığı görülmüştür. Sonuç olarak, bu araştırmada tiazolidinin zebra balığı solungaç ve karaciğer dokuları üzerinde az da olsa zararlı etkilere neden olabileceği görülmüştür.

The potential toxic effects of mancozeb exposure on some antioxidant enzyme were investigated on heart tissue of zebrafish in this study. Zebrafish groups were exposed to different doses of mancozeb (Group A: 5 mgL-1 and Group B: 7.5 mgL-1) for 120 hours. In this study, catalase (CAT) activity, malondialdehyde (MDA) level and total protein (TP) level were determined with spectrophotometer. Our results showed that CAT activity was found 2,541±0,771 mg L-1 in A group and 2,011±0,201 mg L-1 in B group in this study. CAT and MDA activity levels decreased in the experiment group according to control group. MDA levels were found 0,025±0,003 mg L-1 in A group and 0,025±0,003 mg L-1 in B group. TP levels were found 9,75±1,51 mg L-1 in A group and 10,18±0,32 mg L-1 in B group. TP levels increased in the all experiment groups according to control group. We observed that the changes in the CAT activity and MDA levels were time and as well as mancozeb dose dependent. As a result mancozeb is a very toxic substance for zebrafish and other aquatic organisms.