Gly°The following abbreviations are also used: N" for the -lysine derivatives on peptides; Ne for the on peptides. b Descending paper chromatography in solvent VI.e-lysine derivatives 1964). In contrast to the precursor peptide, however, the carbohydrate-free peptide isolated from S. aureus cell walls had only 1 mole of D-alanine, and in addition contained 1 mole of NHS. An Edman degradation carried out on this isolated peptide demonstrated that this ammonia was a substituent of the -carboxyl group of the glutamic acid Tipper et al., 1967a).The studies reported here provide evidence that the basal peptide subunit of the cell wall peptidoglycan of the three Gram-positive bacteria: S. aureus Copenhagen, M. roseus R 27, and Streptococcus pyogenes group A, type 14, has indeed the structure V"-(L-alanyl-D-isoglutaminyl)-L-lysyl-D-alanine.
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