Objectives-To determine the kinetics of elimination of urinary dialkylphosphate metabolites after oral and dermally applied doses of the organophosphate pesticide chlorpyrifos to human volunteers and to determine whether these doses aVected plasma and erythrocyte cholinesterase activity. Method-Five volunteers ingested 1 mg (2852 nmol) of chlorpyrifos. Blood samples were taken over 24 hours and total void volumes of urine were collected over 100 hours. Four weeks later 28.59 mg (81567 nmol) of chlorpyrifos was administered dermally to each volunteer for 8 hours. Unabsorbed chlorpyrifos was washed from the skin and retained for subsequent measurement. The same blood and urine sampling regime was followed as for the oral administration. Plasma and erythrocyte cholinesterase concentrations were determined for each blood sample. The concentration of two urinary metabolites of chlorpyrifos-diethylphosphate and diethylthiophosphate-was determined for each urine sample. Results-The apparent elimination half life of urinary dialkylphosphates after the oral dose was 15.5 hours and after the dermal dose it was 30 hours. Most of the oral dose (mean (range) 93% (55-115%)) and 1% of the applied dermal dose was recovered as urinary metabolites. About half (53%) of the dermal dose was recovered from the skin surface. The absorption rate through the skin, as measured by urinary metabolites was 456 ng/cm 2 /h. Blood plasma and erythrocyte cholinesterase activity did not fall significantly during either dosing regime. Conclusion-An oral dose of chlorpyrifos was readily absorbed through the skin and almost all of the dose was recovered as urinary dialkylphosphate metabolites. Excretion was delayed compared with the oral dose. Only a small proportion of the applied dose was recovered during the course of the experiment. The best time to collect urine samples for biological monitoring after dermal exposure is before the shift the next day. The amounts of chlorpyrifos used did not depress acetyl cholinesterase activity but could be readily detected as urinary dialkylphosphate metabolites indicating that the urinary assay is a more sensitive indicator of exposure. (Occup Environ Med 1999;56:10-13) Keywords: biological monitoring; chlorpyrifos; organophosphorus pesticideThe organophosphate pesticide chlorpyrifos (O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl) phosphothioate) is a non-systemic cholinesterase inhibitor used to combat agricultural and horticultural insect pests. Several pesticide formulations containing chlorpyrifos have been marketed by diVerent manufacturers. These include Dursban, Lorsban, Brodan, Eradex, and Spannit. A study of the metabolism of chlorpyrifos in rats has shown that the major metabolites are diethylphosphate, diethylthiophosphate, and 3,5,6-trichloro-2-pyridinol.

No abstract

No abstract

No abstract

Gender differences in creatinine concentrations and the range of 0.3-3.0 g/l (2.653 and 26.53 mmol/l) traditionally used for confirming acceptability of urine samples mean that 2.5% of samples from male and 9% from female workers were flagged as 'low creatinine' and required a repeat sample. In addition, care should be taken interpreting any apparent gender differences in BM results to ensure that they are due to exposure and not an artefact of creatinine adjustment.