The effect of fertilizing three triticale varieties (Timiryazevskya, Karmen, and Ukro) with nitrogen at different rates either one time at pre-sowing (N30, N 60, N90, N 120 and N150) or two times at pre-sowing and flowering stage (N90+ N30) and (N90+ N60) has been studied. The data indicated that all nitrogen treatments applied either at pre-sowing or/ and flowering stage resulted in a significant increase in triticale yield of three varieties (t/ha) compared to control. The highest yield in Timiryazevskya (5.52 t/ha) was obtained after N120+N0 treatment, while in case of Karmen the highest yield (5.19 t/ ha) was obtained with N150+N0 fertilizing, as to Ukro the highest value (5.05 t/ ha) was noticed after N90+N30 treatment. Besides, the values of 1000 grains weight (gm) insignificantly increased in all cases. Protein and gluten percentage points grew simultaneously with increasing nitrogen amount.