The susceptibility pattern against oxolinic acid of 161 bacterial strains causing urinary tract infections was investigated simultaneously in serial dilution and agar diffusion tests. Oxolinic acid proved to be very active against E. coli and pathogens of the Proteus and Klebsiella-Aerobacter group. Most Staphylococcus aureus strains were also sensitive. The antimicrobial activity against Ps. aeruginosa and Enterococci, however, was low. There was good accordance between the results of the serial dilution tests and of the agar diffusion tests (2 μg sensitivity discs). In addition, the susceptibility pattern of 621 urinary tract pathogens was evaluated in agar diffusion tests. As compared to other widely used antibiotics and chemotherapeutic agents oxolinic acid demonstrated in vitro a favorable antimicrobial activity.

Minimal inhibitory concentrations of amikacin for 296 strains of gram-positive and gram-negative bacteria determined in Mueller-Hinton broth by the method recommended by the International Collaborative Study Group confirmed previous observations that amikacin is effective against current clinical isolates sensitive to gentamicin and that strains moderatly sensitive or resistant to gentamicin are often inhibited by clinically achievable concentrations. A simple linear regression analysis was used for comparison of zone sizes obtained with 10-mug disks of amikacin with minimal inhibitory concentrations obtained by the tube dilution technique. For 152 strains, including seven species of Enterobacteriaceae, the correleation coefficient (r) was -0.79 on Oxoid diagnostic senstivity test agar (which yields zone sizes similar to those obtained on Mueller-Hinton medium) and -0.78 on Oxoid isotonic sensitest agar (which contains cations in isotonic concentrations); the figures were -0.74 and -0.66, respectively, for 80 strains of Pseudomonas aeruginosa. Zone sizes for enteric gram-negative bacilli were on the average 2-3 mm smaller on isotonic sensitest agar than on diagnostic sensitivity test agar, and about 6 mm smaller for strains of P. aeruginosa and Providencia. Minimal inhibitory concentrations of amikacin can be estimated satisfactorily by extrapolation of results of agar diffusion tests, but the composition of the medium, particularly its calcium and magnesium content, should be defined precisely.

Abdruck angeboten werden. -Mit der Annahme des Manuskriptes erwirbt der Verlag die ausschließliche Nutzung an allen Verwertungsrechten im Sinne der 15 ff. des Urheberrechtsgesetzes, insbesondere das Recht der Vervielfältigung und Verbreitung sowie der Übersetzung. -Kein Teil der Zeitschrift darf ohne DEUTSCHE MEDIZINISCHE WOCHENSCHRIFT schriftliche Genehmigung des Verlages über den in D) Alternierender Vergleich von Carbenicillin und Gentamycin bei Kindern dn S 53 und 54 des Urheberrechtsgesetzes gezogenen Rahmen hinaus verwertet werden, ist gewerblichen Unternehmen die Anfertigung einer fotomechanischen Vervielfältigung (Fotokopie, Mikroskopie) für den innerbetrieblichen Gebrauch nach Maßgabe des zwischen der Inkassostelle für Urheberrechtliche Vervielfältigurtgsgebühren GmbH und dem Bundesverband der Deutschen Industrie e. V. abgeschlossenen Gesamtvertrag gestattet, so sind die Gebühren durch Wertmarken zu entrichten. Auf jede vervielfältigte Seite ist eine Wertmarke von DM -,15 zu kleben,

No abstract