Introduction The grey wolf (Canis lupus) is the natural host of many parasites. These animals travel quite long distances to search for prey and nests, causing parasites to spread over large areas; therefore, determination of the parasites carried by grey wolves is important. Methods In this study, we used both morphological and molecular methods for parasitological identification of helminth species. For this purpose, the material obtained after necropsy was examined by macroscopic, microscopic, and molecular (multiplex PCR and DNA sequencing) methods. Results No pathological lesions and parasites were detected in the macroscopic examination of the trachea, lungs, heart, liver, spleen, stomach, and kidneys. The parasites collected from the intestines and diaphragm muscles were identified as Taenia hydatigena, Mesocestoides litteratus and Trichinella britovi. Conclusion The aim of this study was to determine the helminth species in a dead grey wolf from wildlife. To the best of our knowledge, with this study, Taenia hydatigena, Mesocestoides litteratus and Trichinella britovi were detected for the first time in a grey wolf in Turkey.

SummaryThe effects of selamectin were studied in mice naturally infected with the mites Myobia musculi, Myoceptes musculinus and Radfordia ensifera and with the oxyurid nematodes Aspiculuris tetraptera and Syphacia obvelata. The mice were divided into three treated and three control groups (n ¼ 9). Selamectin in the range 10-12.4 mg/kg was applied topically to the skin in a single spot at the base of the neck in front of the scapulae. The mice of treated and control groups were necropsied on the 4th, 7th and 21st day after the treatment. While selamectin was 100% effective in removing M. musculi, M. musculinus and R. ensifera by the seventh day, its effect against S. obvelata and A. tetraptera was 36.7% and 49.2%, respectively on the 21st day.

T h e d e t e r m in a t io n o f im m u n e r e a c t iv e p r o t e in s in C ysticerc u s t e n u ic o l lis c y s t flu id s b y SDS-PAGE a n d W e s t e r n b l o t t in g in Sum m ary:Cysticercus tenuicollis, the larva of Taenia hydatigena, is com m only seen in sheep and goats slaughtered in Turkey. In this study, the protein bands w ere revealed in C. tenuicollis cyst fluid antigen by using SDS-PAGE and W estern blotting, and immune reactive bands w ere determined. Ten positive and 10 negative sera for C. tenuicollis from sheep and one non-infected sheep serum w ere tested in this experiment. A ccord in g to the results, there w as only one protein band determ ined to be immune reactive, w hich w as 3 6 kDa. KEY WORDS : Cysticercus tenuicollis, sheep, SDS-PAGE, Western blotting R ésu m é : R e c h e r c h e d e s p r o t é in e s im m u n o r é a c t iv e s d a n s le LIQUIDE DE KYSTE DE CYSTICERCUS TENUICOLLIS PAR SDS-PAGE ET W e s t e r n B l o t c h e z le m o u t o n

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Altın çakal (Canis aureus Linnaeus, 1758), Türkiye'de bulunan, dünyada da oldukça geniş yayılıma sahip yabani karnivorlardan biridir. Yabani karnivorlar çeşitli zoonoz helmintleri çevreye, insanlara ve diğer hayvanlara bulaştırmaları bakımından önemli bir kaynak oluşturmaktadır. Bu araştırmada, çakallarda bulunabilecek, çevreye bulaşarak insan ve hayvan sağlığını tehdit edecek helmintlerin varlığı aranmaktadır. Bu amaçla, Türkiye'nin Batı Karadeniz ve Güney Marmara bölgesinden Veteriner Kontrol Merkez Araştırma Enstitüsü'ne gönderilen iki çakal incelenmiş, sindirim sistemlerinde; Alaria sp., Joyeuxiella echinorhyncoides, Mesocestoides sp., Uncinaria stenocephala, Rictularia cahirensis erişkinleri ile, Linguatula serrata nimfleri saptanmıştır. Bu çalışmada tespit edilen Alaria sp. ve L. serrata nimfleri bildiğimiz kadarıyla Türkiye'de altın çakallardan bildirilen ilk kayıttır.