RESUMENEl objetivo del estudio fue evaluar el efecto de la congelación en los parámetros morfométricos de los espermatozoides del carnero. Para ello se utilizó el semen de 2 carneros jovenes de la raza Corriedale de 2 y 2.5 años, con una condición corporal de 3.5. Se evaluaron el volumen, motilidad, concentración, viabilidad, integridad de la membrana y la morfometría de los espermatozoides en las muestras. Todas las variables morfometricas se determinaron con un sistema CASA (ISAS, Proiser). El proceso de congelación se realizó con diluyente a base TRIS con 3% de lecitina de soya y glicerol como crioprotector. Se analizaron 12 parámetros morfométricos del espermatozoide, longitud, anchura, área, perímetro, elipticidad, elongación, regularidad y rugosidad de la cabeza del espermatozoide, porcentaje de acrosoma, y la anchura, distancia y ángulo de inserción de la pieza intermedia del espermatozoide de carnero. Se evaluaron una población de 2518 espermatozoides en semen fresco, refrigerado y descongelado. Nuestros resultados indican que algunos parámetros morfométricos del espermatozoide (longitud, anchura, área y perímetro de la cabeza) criopreservado son de menor dimensión (p<0.05) a lo encontrado en semen fresco y refrigerado.Palabras clave: CASA, morfometría espermática, criopreservación, semen carnero ABSTRACTThe objective of the study was to evaluate the effect of freezing on the morphometric parameters of ram spermatozoa. For this purpose, the semen of 2 young rams of the Corriedale breed of 2 and 2.5 years old was used, with a body condition of 3.5. The volume, motility, concentration, viability, membrane integrity and sperm morphometry were evaluated in the samples. All morphometric variables were determined using a CASA system (ISAS, Proiser). The freezing process was performed with TRIS based diluent with 3% soy lecithin and glycerol as cryoprotectant. A total of 12 morphometric parameters were analyzed (length, with, area, perimeter, ellipticity, elongation, regularity and roughness of head spermatozoa, acrosome percentage, and the width, distance and insertion angle mid piece ram sperm). A population of 2518 spermatozoa was evaluated in fresh, refrigerated and thawed semen. Our results indicate that some cryopreserved sperm morphometric parameters (length, width, area and perimeter of the head) are smaller (p <0.05) than that found in fresh and refrigerated semen
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.