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The influence of adsorption and multiplicities of infection on the transducing system Proteus niirabilis--n, was studied. The frequency of transduction and the rate of survival depended on the duration of the incubation of the transducing mixture. The maximum of transducing frequency was obtained after 5 min of incubation a t 37 "C. This time corresponds t o that required for optimal adsorption. The frequency of transduction and the rate of survival depended on the multiplicities of infection. An optimal frequency of transduction for the system studied was obtained with multiplicities of infection ranging from 4:l to 8:l. Die Transduktionsbaufigkeit ist in allen bekannten

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The influence of different niult~iplicities of infection on the formation of infectious and transducing n,-phages was studied. The ratio of the two types of n,-phages formed is not the same. An agent is described which killed P . mirabilis bacteria without forming plaques.The synthesis of this agent is correlated with the formation of transducing phages, but not that of infectious phages. The possible identity of this agent with phage structures able to kill bacteria without plaque formation is discussed.