The purpose of this study is to examine and analyze the influence of accountability and transparency principles, open, fairness and competition against e-procurement effectiveness and efficiency in procurement service unit Maluku Province. The population of this study was all participants or providers of government Goods/Services registered in procurement service unit Maluku Province with purposive sampling as a sample method with 79 participants or providers of government Goods/Services as respondents. Data collection was done by direct survey. Hypothesis testing was empirically tested using Multiple Linear Regression Analysis. The results showed that the principle of accountability and transparency, openness, fairness / non-discrimination and competition proved to have a positive and significant impact on the effectiveness and efficiency of e-procurement in procurement service unit Maluku Province. This can be proven through regression coefficient indicating that if the principle of accountability and transparency, openness, fairness / non-discrimination and competition increase, then it will be followed by improvement of effectiveness and efficiency of eprocurement at procurement service unit Maluku Province. Thus, it can be said that increasing the principles of accountability and transparency, openness, fairness / nondiscrimination and competition will improve the effectiveness and efficiency of e-procurement in procurement service unit Maluku Province.