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Introduction: A huge reservoir of bioactive compounds exists in many species of plants, only a small percentage of which have been examined and continued to be an important source of anticancer agents. Worldwide effects are ongoing to identify new anticancer compounds from plants.With the current decline in the number of new molecular entities from the pharmaceutical industry, novel anticancer agents are being sought from traditional medicines. Objective: In the present study we investigated the efficacy of methanol extracts of Pavonia odorata, for its clonogenic inhibition on Human Breast cancer (MD-MB-231), Prostate cancer (PC-3) and Lung cancer (Calu-6) cell lines. Materials and Methods: The cytotoxic effect was evaluated by MTT assay. Results: The methanol extract of P. odorata showed significant cytotoxicity against MD-MB-231 and Calu-6, when compared to PC-3cells. Conclusion: The methanol extracts of P. odorata, showed effective cytotoxic activities in a dose dependent

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