The ever-increasing harmful effect of endo- (products of inflammatory processes of genital tissues) and exogenous factors (environmental conditions) on the body of women and animals (cows) leads to a substantial decrease in ova fertilisation during mating and artificial insemination. Therefore, the purpose of this study was to identify changes in the signs of physical condition (colour, consistency, fluidity, impurities, etc.) and ratios (homeostasis) of H2O mass, organic (OS) and inorganic (IS) substances of uterine-vaginal mucus (UVM) should be used to assess their harmful effect on the fertilisation of cows after their first insemination. The following methods were used for this study: ocular estimate of the physical condition of freshly obtained mucus; gravimetric assessment of the features of changed mass parameters of its dry residue (DR) components; mathematical and statistical analysis of certain indicators. Determined by the gravimetric method, the features of the imbalance of mass (g, mg), its distribution (%) and ratios (Im:1, Ic:1) in the system of the “medium – substance” type due to the harmful effects of endogenous factors (products of inflammatory processes) indicate that the secretions of the genitals released during oestrus are characterised not only by changes in signs, but also ±2–4 times different from the norm (control) indicators of the mass of components. Under such circumstances, the distribution of substances in the DR samples of the experimental group is expressed by the series, where the mass OS1˃IS˃OS2, but in the control group – OS1˃IS˃OS2. This means that the products of inflammatory processes released by the genitals of cows change the norm of homeostasis of the mass (distribution of components) of OS and IS of the biological system of the “medium – substance” type. Indicators of the obtained mass ratios between component pairs H2O:DR; H2O:OS2, H2O:OS1, H2O:IS (control group – 63:1, 586:1, 231:1, 109:1; experiment group – 42:1, 310:1, 87:1, 112:1), except for the H2O:IS pair (P˂0.05), indicate the presence of probable changes in the homeostasis of OS and IS in the “H2O – components” system. The high probability (P˂0.02; ˂0.001) of the detected changes is inherent in the “DR – components” system, namely: if the average ratio indicators of pairs DR:OS2; DR:OS1; DR:IS samples of the control group are 9:1, 4:1, 2:1, then the same indicators in the experimental group are 7:1, 3:1, 2:1, respectively

To substantiate the possible relationship of the parameters of homeostasis of the concentration of Ca2+, K+, Na+ and the mass of organic and inorganic substances secreted synthesized еt by the tissues of the genital system of males and females (bull – sperm plasma; cow – vaginal mucus), with passive and/or by active methods of movement of sperm and ova through their channels and ducts, the peculiarities of the distribution of components of open and closed systems of the type "environment – cell (substance)" were investigated. Methods of spectrophotometric (flame photometry, Flapho-4) and gravimetric (combustion, extraction, elimination) analysis of the studied samples experimentally established that the adaptive response of sperm to the protective and/or harmful effects of extreme conditions (concentration, temperature, properties of protective environment components). Sperm preservation (TCS) is different. Depending on those (exo-) or other (endogenous) circumstances, the antiportic way of moving ions can change to symportic. The peculiarities of the parameters of concentration and mass of the components of the studied systems revealed in this connection indicate that the fractions of aqueous extracts (PVE) of tissues and secretions of bull and cow, at different functional states of genitals (norm, pathology), have different concentrations of free of bound alkali metal ions, mass and content of substances of their dry residue eliminated in distilled water and insoluble (water, acids). The limit of the difference of the ratio of the ratio (IC:1) of the concentration of one- (Ca2+:Ca2+, K+:K+, Na+:Nа+) and different (Na+:Ca2+, K+:Ca2+, Na+:K+) pairs of ions indicates that that the limit of osmotic pressure parameters of the organic and inorganic components of samples of tissues of genitals, sperm and uterine-vaginal mucus can also be different. This suggests that different parameters of osmotic pressure of the components of the synthesized secretions and the formed sperm and eggs create (initiate, stimulate, inhibit) favorable conditions for their passive and/or active movement through the channels and ducts of the genitals. However, it should be added that the proposed hypothesis requires discussion and experimental confirmation.