Crop production and plant diversity are low due to short development period and low temperature in highlands. This research was conducted to determine the effects of different nitrogen doses on yield and some traits of proso millet (Panicum miliaceaum L.) in altitude of 1860m Eastern Anatolia conditions. The experiment was established in a randomized complete blocks design with four replications in 2013 and 2014. Five nitrogen doses (0, 30, 60, 90 and 120kg N ha-1) were used in this study. Plant height, dry matter yield, crude protein content and yield, ADF and NDF content, biological, seed and straw yield, harvest index and 1000-seed weight were evaluated. In summary, this 2-year study clearly showed that irrigated proso millet can be successfully produced in Eastern Anatolia conditions. The results indicated that nitrogen doses had significant effects on the yields and increased the plant height, dry matter yield, crude protein content and yield, biological yield, seed yield and straw yield. The highest dry matter yield (4640kg ha-1) and seed yield (2699kg ha-1) were determined in 90kg N ha-1 , whereas the highest biological yield (7466 kg da-1) was determined in 120kg N ha-1. 