Background: The role of the nurse is very important in breaking the chain of infection, and they can do this by applying proper infection control practices. The study aimed to assess the knowledge and practice of outpatients Nurses about infection control. Subjects &Methods: Descriptive research design was carried out in this study at Ibn seena hospital, Almukalla and University Hospitals for Maternity and childhood that located in Almukalla city. The total number was 50 nurses selected as a convenient sample. Two tools were used in data collection The First tool is the structured questionnaire it content Participants' knowledge related to nosocomial infection the Second tool was the observation checklists to assess the nurses practice. Results: revealed that 80% of the studied sample has not attended seminars or conferences about infection control and 54% of them was given vaccine for hepatitis, and 90% had poor knowledge and practice. There was no statistically significant difference between knowledge and practice Conclusion: It is concluded that the studied nurses had poor knowledge and practice. Also, there is positive correlation between knowledge and practice about infection control. Recommendation: Should be apply in services programs about infection control to all nurses in outpatient's clinics, also establish national guidelines for infection control practice in outpatient clinics.