Poultry meat is the main and cheap source of animal protein and fulfilling the protein requirement of growing population of the world. However, maximum growth of broilers is obtained by supplementing various nutrient through diet both from natural and artificial sources. Amino acids are one of the major nutrients required for optimum growth of broilers. Amino acids are building blocks of protein and regulate different pathways which are necessary for growth, maintenance, feed conversion ratio (FCR) and immunity in birds. Amino acid in the diet of broiler belong from both natural and artificial sources. Among amino acids required for growth and immunity of broilers arginine is an essential amino acid that should be supplemented through diet. This review will provide the insight that how different levels of arginine influence both performance parameter and immunity in the broilers. Based on the review we concluded that arginine level of 1.20-1.25% during starter phase and 1.10% during finisher phase is essential to optimize the performance; however, arginine level of 1.20% in the broiler diet is optimum to maintain antibiotic titer of bird that will help to resist birds against the different infectious disease. Therefore, it is recommended that arginine level should be maintained in the diet for optimum performance and immunity in broilers.