Objective: In this study，we aimed to identify differentially expressed heat shock proteins (HSPs) profile in the villi and decidua from early missed abortion(EMA) patients. Methods: By using high-throughput and high-precision Parallel Reaction Monitoring (PRM)-based targeted proteomics technique, this study studied the expressions of HSPs in villi and decidua of 10 EMA patients and 10 controls. Meanwhile, the expressions of 3 HSPs in villi of another 22 EMA patients and 22 controls were verified with Western blotting and immunohistochemistry (IHC). Results:Compared with the control group, there were potential differences in the expressions of 16 HSPs and 42 polypeptides in human villi and decidua. Among them, HSP90AB1, HSPD1 and HSPA13 were down-regulated in expressions in villi of EMA patients, with a statistically significant difference, which was consistent with the verification results of Western blot and IHC. Conclusion: Using PRM- based targeted proteomics technique, this study for the first time screens and quantitatively analyzes the expression profile of HSPs in villi and decidua of patients with EMA. The significant down-regulated expressions of HSP90AB1, HSPD1 and HSPA13 are discovered to have a potentially intimate association with the occurrence of EMA. Findings in our study may provide novel potential research targets related to HSPs for the pathogenesis, prevention and treatment of EMA.

Objective In this study, we aimed to identify differentially expressed heat shock protein (HSP) profiles in the villi and decidua from patients with early missed abortion (EMA). Methods By using high-throughput and high-precision parallel reaction monitoring (PRM)-based targeted proteomics techniques, this study examined the abundance of HSPs in the villi and decidua of 10 patients with EMA and 10 controls. Moreover, the abundance of 3 HSPs in the villi of another 22 patients with EMA and 22 controls was verified with Western blotting and immunohistochemistry (IHC). Results There were potential differences in the abundance of 16 HSPs and 42 polypeptides in human villi and decidua compared with those of the control group. Among them, HSP90AB1, HSPD1 and HSPA13 were downregulated in abundance in villi of patients with EMA, with a statistically significant difference, which was consistent with the verification results of Western blots and IHC. Conclusion Using a PRM-based targeted proteomics technique, this study is the first to screen and quantitatively analyze the expression profile of HSPs in the villi and decidua of patients with EMA. The significant downregulation of HSP90AB1, HSPD1 and HSPA13 was found to have a potentially intimate association with the occurrence of EMA. The findings in our study may provide novel potential research targets related to HSPs for the pathogenesis, prevention and treatment of EMA.