Tilia amurensis is one of the most valuable broad-leaved trees in northeast China. It is very important to study cutting because T. amurensis seeds have dormancy characteristics, which caused a great obstacle for its propagation. In this study, the effect of different factors and levels on rooting rate of T. amurensis cuttings were investigated by a orthogonal design (L1645). The age of cutting, hormone concentration and matrix all affected rooting rate, among which matrix was the main factor, followed by ages of the mother tree and the hormone concentration. From three to four years old cuttings treated with a hormone concentration of 1.0 × 10-4 and cultured in vermiculite and sand could get higher rooting rate. The best combination of treatments was to use 3-4a aged cuttings treated with IAA(1.0×10-4) and dig into the sand for 16h, which rooting rate got to 63.3%.
The influence of the preparation conditions of the impregnation-vacuum filtration method was investigated systematically on the Ni dispersion and the activity of Ni-cordierite structured catalysts in hydrogenation of m-dinitrobenzene to m-phenylenediamine. H2-TPD measurement showed that the Ni dispersion has close relationship with the impregnation solution concentration of nickel nitrate, the impregnation time, the vacuum degree, the vacuum filtration time and the calcination temperature. The hydrogenation activity test and nitrogen physisorption investigation showed that the catalytic performance of Ni-cordierite is dependent upon the Ni dispersion and the chemisorption mode of m-dinitrobenzene on Ni particles.
This paper proposed an orthogonal design L27(313) with four factors(seeds shucked off pericarp, dipped in water, GA3 treatment, 6-BA treatment and stratification)and three levels based on the dormancy of Tilia amurensis seed. The optimal way for quikly breaking seed dormancy was obtained. That was: 1)shucking off the pericarp; 2)scalding seeds for 2s with 100°C boiling water, then soaking in room temperature water for 24h; 3)soaking in 100 mg•L-1 GA3 and 6-BA solution, respectively for 24h; 4)stratification in varying temperature (10-15°C)for 5d, then in low temperature(0-5°C)for 15d. The germination rate of T. amurensis seed could get to 70.2% by above treatment and germination time was shortened to 30 days which was greatly improved compared to previous study.
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