Secondary metabolites (SM) products permanently played an important role in medicine; fungi metabolites have increasingly become major players in recent pharmaceutical discovery in particular antimicrobial agents. The aim of study was to prepare and characterize of potential biological extract of culture filtrate of Trichophyton rubrum and tested as antibacterial, antioxidant agents. Local isolates of a dermatophyte T. rubrum were used and examined for production of SM. Extracts were checked for physical and chemical characterization using Fourier transform infrared spectrophotomete and HPLC and their cytotoxicity using a sensitive in-vitro brine shrimp lethality bioassay. The results revealed the isolation, preparation and characterization of different components of SM, included especially kojic acid, fusidic acid, amides and sulfones which gave antibacterial and antioxidant properties. In conclusion, the current study established the ability of a dermatophyte, T. rubrum to produce antibiotic-like substances, especially the sulfones which not isolated and recorded previously from T. rubrum.

Breast cancer is one of the most dangerous and most common malignanciesamong women in the world, which affects different age groups of women, IL-17 plays an important role in chronic inflammation and cancer, Serum IL-17BR concentration which useful in early diagnosis and staging of breast tumors.The aim of the study was to evaluate of IL-17BR serum concentrations in patients with benign and malignant breast tumors and study the relation between the above parameter and breast cancer development. The study population was composed of 120 samples 24 patients with recently diagnosed breast carcinoma,6 patients with repetitive carcinoma,40 patients with benign breast tumor (fibroadenoma) and 50 normal apparently health woman as a control.ELISA technique was applied for estimation of IL-17BR levels in patients as well as apparently healthy volunteers of women. The results revealed the mean age of malignant breast females was 52.8 ± 12.3 (Mean ± SD),while it was 26.9 ± 8.3 years for women with benign tumor with highly significant difference(P<0.001),IL-17BR level was determined and found that was a highly significant difference in its level among benign breast females subjects (72.48 Ng/ μL) and healthy control (50.87 Ng/ μL) P< 0.001),no significant difference in the mean of sIL-17BR level among the different patients‘ groups (49.83,64.33 Ng/ μL) for recurrent and primary breast cancer respectively.Estimation of IL-17BR level showed significant elevation of the concentration among the sera of recurrent breast cancer in comparison with other groups which perhaps regarded as a prognostic marker.

Tuberculosis (TB) disease and Rubella virus adversely affect Latent Tuberculosis Infection (LTBI) in pregnancy and major cause of maternal-child mortality. The aim of this study was undertaken to perform cross-sectional research to evaluation agreement and a correlation between an IFN-γ releasing assay for detected latent Tb infection and anti-rubella antibodies tests in pregnant women with recurrent miscarriages.Method: the study was carried out between 1 March 2018 Until 1 July 2019.Study groups: A total of 75 pregnant women living in TB-affected areas were in direct or indirect contact with an infected person from their families or relatives and had a history of recurrent miscarriage in the first months of pregnancy, their ages ranged from (18-41) years. There screened for Rubella IgG and IgM and IFN-γ release assays (IFN-γ RAs). The research showed a following results, Anti-Rubella IgG, IgM (positive) according to recurrent miscarriage, number and percentage of Rubella IgG, IgM positive was (36)(48%);(22)(73.3%), (4)(5.3%); (0)(0.0)in both groups(study and control) respectively, and significant P value> 0.05. The Quantiferon test in different study group and control group according to age groups showed that out of recurrent miscarriage pregnant women have positive result 4 (5.33). Comparison the results of two tests of Quantiferon release IFN-γ assay and Anti-rubella virus antibody (IgG, IgM) that had significant relationship P= 0.0443. Conclusion of our findings indicates whether any of these tests should be done and how sensitive they are a great difference in test results when used between recurrent miscarriages pregnancy.