Objectives: The present research aimed to find an association between infection by  Helicobacter pylori and vitamin D deficiency in type 2 diabetes mellitus among Iraqi individuals attending Al-Yarmouk Teaching Hospital. Methods: According to fasting blood glucose,  the samples were divided into a non-diabetic group with ten diabetic individuals and a diabetic group with thirty individuals. Results: The anti-H. Pylori (IgG) levels were 86.77± 58.62 NTU/µL in diabetic patients compared with 10.12 ± 7.40 NTU/µL in non- diabetic group. Vitamin D levels were decreased significantly in infected patients compared to non-infected subjects. Conclusion: The H pylori-infected patients have recorded the lowest level of vitamin D than non-infected individuals.