One of the ecosystems inhabited by many types of fish is a river. Kuning River is one of the rivers in Sleman Regency, D.I. Yogyakarta, which upstream is located at Mount Merapi. In 2010 there was a large cold lava flood in the Yellow River from Mount Merapi. So far, no identification of fish species in the river has been carried out. The purpose of the study was to determine the types of fish in the Yellow River (both local and introduced) and the state of the abiotic environment. The method used is purposive sampling with three research stations. Each station is divided into three sampling points. The fish samples obtained were identified in the Biology laboratory of Ahmad Dahlan University. Based on the identification, there are a total of 16 species of fish, with 13 local species, namely; Barbodes binotatus (cakul), Hampala macrolepidota (palung), Mystacoleucus obtusirostris (kepek), Rasbora argyrotaenia (wader pari), Labiobarbus leptocheilus (lukas), Barbonymus gonionotus (tawes), Osteochilus vittatus (nilem), Nemacheilus fasciatus (kepala timah), Channa gachua (kotes), Channa striata (gabus), Trichopodus trichopter (sepat), and Dermogenys pusilla (cucut tawar) and 3 introducedspecies, namely; Poecilia reticulata (gupi), Xiphophorus helleri (ekor pedangl), and Amphilopus labiatusus (red devil). Abiotic parameters in the Yellow River, Sleman Regency, DIY; average temperature, water pH, current strength, dissolved oxygen (DO) indicate normal conditions for fish survival.