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The layered composite rock was subjected to triaxial compression tests under constant confining pressure and the stress–strain curves under different confining pressures were obtained. Based on the continuous damage theory and statistical strength theory, it is assumed that the strength of rock microelements obeys Weibull distribution by taking the defects such as random micro-cracks in the rock into account. The statistical constitutive model of layered composite rock with damage correction is established by taking the axial strain of rock as a random distribution variable of microelement strength. The model parameters were determined by the curve fitting method and referring to some test parameters. By comparing the experimental data and the constitutive model curve, the rationality and feasibility of the model are verified.

Aims:  The impact of DNA extraction methods on biological activated carbon (BAC) DNA yield and bacterial community was evaluated. Methods and Results:  Three different DNA extraction methods were compared: method a, method b and method c. Method c with ultrasonic pretreatment improved cell lysis efficiency (from 34% to 87%) and DNA yield [from 10·58 μg g−1 (dry wt) of carbon to 21·42 μg g−1 (dry wt) of carbon]. denaturing gradient gel electrophoresis profiles obtained by method c recovered the five seeded bacteria (Bacillus subtilis Strain WSO 6, Pseudomonas putida Strain WSO 7, Acinetobacter lwoffii WSO 10, Pseudomonas pertucinogena WSO 11 and Brevibacterium mcbrellneri WSO 13). Conclusions:  The results showed method c with ultrasonic pretreatment was the most successful for the analysis of BAC bacterial community because it was effective in the detachment of bacteria and cell lysis, thereby resulting in good yields. Significance and Impact of Study:  These results must be taken into consideration when extracting DNA for analysing BAC bacterial community.

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