The efficiency of DNA extraction from whole blood using appropriate method is very important for molecular analysis. Therefore, the aim of this study was to compare the purity and concentration of DNA extraction method from bovine (Bos taurus), chicken (Gallus gallus), and porcine (Sus scrofa) blood. The DNA of blood samples was extracted using three types of kit, namely InnuPREP Blood DNA Mini Kit, Wizard Genomic DNA Purification Kit, and QIAamp DNA Blood Mini Kit. The results showed that blood DNA extracted using QIAamp DNA Blood Mini Kit was found to be the most effective and consistently produced high concentrated and pure DNA for three animal samples. The purity of DNA ranged from 1.73 ± 0.05 Å to 1.94 ± 0.21 Å and the range of blood DNA concentration extracted using the QIAamp DNA were between 13.73 ± 2.11 and 25.01 ± 2.08 ng/?l. However, the blood DNA of porcine was not successfully extracted using InnuPREP Blood DNA Mini Kit and Wizard® Genomic DNA Purification Kit. These results were very crucial for the subsequent use of amplification using polymerase chain reaction (PCR) and to facilitate accurate detection in further analysis.
Arabian Yogurt and White Cheese is a popular food that contains various probiotic lactic acid bacteria (LAB). This study was conducted to evaluate the effect of Arabian yogurt and white cheese on some physiological parameters which have benefits to health of human. 75 volunteers were selected and divided into 3 groups the volunteers were given yogurt and white cheese every morning as a breakfast meal for (21) days. Blood samples were collected at the end of the experiment. The results in this study exhibited a significant decrease (P<0.05) in the volunteer weight, also in the concentration of serum cholesterol, Triglyceride, LDL-C, and VLDL-C, uric acid, and glucose, on the other hand significantly increased (P<0.05) in HDL-C concentration in the two groups, besides the study, relived no significant decrease in Hb, PCV, and WBC. This study showed no significant variations in the urea of volunteers. The data indicated that Yogurt and White Cheese contains various LAB species that are potential probiotic candidates with health benefits."
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