Cases of inappropriate sexual behaviours have been on the increase in Nigerian society and they transcend age limits and gender of the victims. This study examined the impact of sexual harassment on the overall well-being of female senior non-teaching workers of public tertiary institutions in Ogun State, Nigeria. Three research hypotheses were formulated and a descriptive research design was adopted for the study. The population used was 150 female senior non-teaching workers from the selected public tertiary institutions in Ogun State, Nigeria. Purposive and simple random sampling techniques were used to select 50 samples from each of the selected tertiary institutions. The data collected was analysed using descriptive statistic of frequency and percentage while inferential statistics was used to test the hypotheses. The findings of the study revealed that sexual harassment has a significant impact on the overall well-being of female senior non-teaching workers. Based on the findings of this study, it was recommended that the proprietors of these public institutions should make relevant legislation and regulations to guide against this as there seems to be no specific punishment for sexual harassment in the code of conduct of these institutions aside from lumping it up under general misconduct.