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By use of the spllnes-under-tenslon concept, a simple algorithm has been developed for the three-dimensional representation of nonuniformly spaced data. The representations provide useful Information to the experimentalist when he Is attempting to understand the results obtained In a self-adaptive experiment. The shortcomings of the algorithm are discussed as well as the advantages.

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mismatch to zero may be due to unknown impurities or a mechanism other than the classical competitive type.From the 1/V0 vs. [I] replot (Figure 12), a good estimate of X; ~0.45 mmol/L can be obtained which agrees well with Xmipp at [I] = 0 (Figure 13). Note, the plot is nearly perfect. Only one line, corresponding to 0.50 mmol/L substrate, is not confluent with the other 20 lines. The above are only a few examples of how response surfaces, coupled with traditional plots and replots, can be used to gain an understanding of enzyme catalyzed reactions. CONCLUSIONSMany kinds of chemical experimentation can be greatly improved by the use of the appropriate instrumentation and computers on-line to the experimental apparatus. These systems can provide many times the quantity and quality of data obtainable by manual methods. In addition this apparatus enables the experimenter to perform experiments that are otherwise impossible or extremely difficult and time consuming. Further improvements in experimental capabilities are obtained when interactive graphics are used on-line as an aid for interpretation of the vast quantities of data that are generated by an automated system.We have described an apparatus with these attributes. The system is being used to study enzyme mechanisms, optimize analytical methods, and develop new experimental and control techniques. Data obtained from the alkaline phosphatase enzyme-catalyzed reaction was used to show the versatility and power of apparatus and evolving graphic techniques.

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