No abstract

Helicobacter infection is a major public health issue worldwide, about 4.4 billion individuals worldwide were confirmed to be infected. Helicobacter is a human pathogen that is transmitted from human to human and has a zoonotic relationship. Helicobacter gastritis, peptic ulcer, and perforation may lead to cancer. This study aims to try to isolate the Helicobacters from gastric biopsies and autopsies in human patients and animals (dogs and cats). Methodology:105 gastric biopsies were collected from dyspeptic patients and 5 gastric autopsies were collected from dogs and cats. The samples were cultivated and characterized by microbiological and molecular tools (PCR). Results:The results showed few harvested isolates (n=22) after characterization the confirmed Helicobacter isolates were 10 only from humans and animals. Conclusion:The conclusion is the microbiological method is so costive, timeconsuming, and less sensitive. The presence of accurate and rapid tests is so crucial for specific and sensitive detection of Helicobacters.

Helicobacter pylori is a zoonotic gut pathogen that affects humans and animals. Half of the world's population is infected with H. pylori. Helicobacter pylori stool antigen lateral flow immunochromatographic assay is the most common rapid test for disease diagnosis. This study aimed to evaluate the commercial Helicobacter pylori stool antigen lateral flow immunochromatographic assay using a gold standard test, a Hemi-nested 16srRNA PCR. Methodology: 200 stool and fecal samples were collected from dyspeptic humans, dogs, and cats. HpSA-LFIA and 16srRNA Hemi-nested PCR were performed on the collected samples. Statistical analysis was applied to get the sensitivity and specificity of the test under evaluation. The sample size was calculated with a power of 80% (online calculator). The stool and fecal samples were preserved at -20°C until the test performance. Results:The obtained Se and Sp were 91.89% and 59.51%, respectively. Conclusion: HpSA-LFIA in our country is a highly sensitive test with low specificity and low accuracy to be the sole test for diagnosis. The test was intended to be used as a screening test and provided a preliminary result which was not enough for precision and final diagnosis.