The main aim of this study was using of Commiphora myrrh extract as a sterilizer against pathogenic microbes isolated from beauty salons. The efficiency of C. myrrh extract was tested against each type of bacteria and fungi that were obtained from the previous study and compared with the efficiency of the chemical reagent used in salons. There were no significant differences in the efficiency of similar efficiency C. myrrh and barbicide solution on the tested microorganisms. The result also revealed that C. myrrh extract had higher antibacterial activity against bacterial isolates than fungal isolates.