Heterosis or hybrid vigor is the improved performance of a desirable quality in hybrid progeny. Hybridization between high-productive Assam type and high-quality Chinese type clone-cultivar is expected to develop elite tea plant progenies with high quality and productivity. Comparative transcriptomics analyses of leaves from the F1 hybrids and their parental clone-cultivars were conducted to explore molecular mechanisms related to catechin content using a high-throughput next-generation RNA-seq strategy and high-performance liquid chromatography (HPLC). The content of EGCG (epigallocatechin gallate) and C (catechin) was higher in ‘Kiara-8’ × ‘Sukoi’, ‘Tambi-2’ × ‘Suka Ati’, and ‘Tambi-2’ × ‘TRI-2025’ than the other hybrid and clone-cultivars. KEGG (Kyoto Encyclopedia of Genes and Genomes) and GO (Gene Ontology) analysis found that most pathways associated with catechins content were enriched. Significant differentially expressed genes (DEGs) mainly associated with phenylpropanoid, flavonoid, drug metabolism-cytochrome P450, and transcription factor (MYB, bHLH, LOB, and C2H2) pathways appeared to be responsible for the high accumulation of secondary metabolites in ‘Kiara-8’ × ‘Sukoi’, ‘Tambi-2’ × ‘Suka Ati’, and ‘Tambi-2’ × ‘TRI-2025’ as were detected in EGCG and catechin content. Several structural genes related to the above pathways have been obtained, which will be used as candidate genes in the screening of breeding materials.

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ABSTRAKStep I: the pBY520 and pRP9 were cut with BamHI and HindIII, and electrophorated with 1% agarose gel. DNA fragments of HVA1 and pRP9 were purified, ligated with T4 DNA ligase, and transformed into Escherichia coli DH5-α by heat shock. E. coli were grown onto solid medium (+ kanamycin 100 mg/l). A new plasmid DNA was isolated from single colony culture of the bacteria, confirmed, and named pRP9_HVA1.Step II: DNA of pRP9_HVA1 and pAY560326 were cut with XhoI dan SpeI enzymes, purified, and ligated. The next procedure was similar to step I, and the resulted plasmid was confirmed by PCR and digestion with XhoI dan SpeI enzymes, and named pAY_HVA1.Step III: pAY_HVA1 was first transformed into Agrobacterium EHA-105 and then into rive varieties Ciherang and Inpari 6 using the early infection of scutellum transformation method. Nine transgenic rice lines that positively contain HVA1 were obtained.

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