Background: The risk rate for the incidence of any mental disorder is 50%, and the prevalence of mental disorders is increasing. This study aimed to evaluate the psychometric of the Iranian Version of Mental Health Literacy Scale(MHLS).Methods: This cross-sectional study was conducted with a multi-stage sampling method with the participation of 1363 individuals in the general population. The formal, content and construct validity were used for validation of MHLS. Cronbach’s alpha coefficient was used for the reliability of MHLS. Confirmatory factor analysis(CFA) was performed using AMOS software Version 24.Results: After performing the CFA, the final version of the MHLS included a total of 29 items, which consisted knowledge of where to seek information (4 item), Ability to recognize disorders (8 item), knowledge of self-treatment (2 item), knowledge of risk factors and causes (2 item), Attitudes that promote recognition or appropriate help-seeking behavior (10 item), and knowledge of professional help available (3 item). In the CFA test, the six items were deleted.Conclusion: Due to the lack of appropriate tools for measuring health literacy in the Iranian population, the 29-item MHLS with six subscales is a valid and reliable tool for measuring mental health literacy in the population.