A young woman with symptoms similar to common cold was referred to Vasei hospital of Sabzevar. The patient had travelled to Malaysia which was mentioned in her history. She hospitalized with symptoms of high fever, headache, and lethargy. Then, she underwent supportive therapy. In next day, the symptoms such as bone pain, myalgias, shortness of breath, abdominal pain, severe headache, eye strain and maculopapular lesions along with nausea, vomiting, and diarrhea were appeared. Laboratory findings showed high grade fever, leukopenia, and severe thrombocytopenia. Considering the findings, dengue fever was diagnosed and the patient serum sample was sent to a reference laboratory for presence of Immunoglobulin M (IgM). The aim of this study was to introduce a new case of dengue fever in Iran.

Background: Breast cancer is the most common cancer in women in most areas of the world. In Iran and Guilan province, frequency of this type of cancer has shown an increasing rate according to statistics of health centers of province in recent years. Despite of efforts on early diagnosis and treatment, this type of cancer is still the second leading cause of deaths due to cancer in women. Objectives: This study aimed at obtaining protein biomarkers that may be useful in diagnosis and treatment of breast cancer. Patients and Methods: In this experimental study, 10 patients with proved breast cancer and 10 patients with healthy breast, healthy and cancerous tissue samples were obtained to perform proteomics. All tissue proteins were purified using standard separation methods and proteins were separated using two-dimensional electrophoresis. Healthy and tumorous tissue proteomes were compared and the extent of protein expression was analyzed. Results: Four hundred fifty four proteins were recognized, 138 of which were identified with changes in protein expression while cancerous. The expression of 61 proteins in tumorous group was suppressed. Three proteins showed increased expression while cancerous and 74 proteins showed decreased expression in cancerous tissue. Conclusions: It seems that in this disease, the expression of a large number of proteins in cancerous tissues changed, most of them decreased or not expressed at all. These changed proteins can be effective as biomarker in diagnosis and treatment of this disease.

No abstract

Background: Human T-cell lymphotropic virus type 1 (HTLV-1) is a human retrovirus that causes two distinct diseases, adult T-cell leukemia (ATL) and a progressive and degenerative myelin disorder called myelopathy associated with HTLV-1 (tropical spastic paraparesis). Similar to human immunodeficiency virus (HIV), infections caused by HTLV-1 and-2 and retroviruses are persistent and long-lasting. Less than 5% of these infections are HTLV-related illnesses, but their treatment modalities are rare and their prognosis is poor. They are often fatal. Patients with β-thalassemia major are clearly at higher risk for HTLV-1 infection due to the need for frequent blood transfusions. Objectives: The current study aimed at detecting the molecular screening for HTLV-1 infection based on fluorescent probe in patients with β-thalassemia. Methods: The current experimental study was conducted on 80 blood samples collected from patients with β-thalassemia major in Shahid Rajaee Hospital of Tonekabon, Iran. After DNA extraction by the Iranian kit, molecular biological system transfer (MBST) Cinna Gen company (Tehran, Iran), TaqMan real-time polymerase chain reaction (RT-PCR) was employed to detect DNA HTLV-1 genome and a tax gene-specific integrated with the genome of patients' lymphocyte was obtained. Results: Results of TaqMan RT-PCR indicated that out of 80 patients with β-thalassemia major referred to Shahid Rajai'e Hospital, two (2.5%) had HTLV-1. Conclusions: Compared to other molecular and serologic techniques, HTLV can be applied to detect HTLV-1 in blood banks due to its sensitivity, simplicity, higher speed, and the ability to simultaneously diagnose HTLV and lower costs than that of fluorescence molecular methods.

Background: In cervical cancer, more than any other type of cancer, the effects of prevention, early diagnosis and prompt treatment is apparent on reduction of mortality. Objectives: This study aims to achieve protein biomarkers which can be useful in diagnosis and treatment of cervical cancer. Methods: In this Descriptive study, out of 3 patients who had a positive HPV PCR test, with symptoms of cervical tumor, samples of healthy and cancerous tissues were obtained to perform proteomics. By standard separation techniques, total tissue proteins were purified and separated by using two-dimensional electrophoresis. Results: Proteomes of healthy and cancerous tissues were compared with each other and the level of expression of considered proteins was evaluated using the required analyses. A total of 173 proteins in gels of two-dimensional electrophoresis was determined, 37 of which had a significant difference in expression. Because descriptive essay. We compared and statistical tests do not make sense. Conclusions: It seems that in this disease, the expression of a large number of tissue proteins in cancerous tissue is changed, and most of them reduced or not expressed at all. These changed proteins can be useful as a marker in identification and treatment of this disease.