The diversity of stain fungi is important if wood is inhabited with various fungi, and the discoloration mechanism will be better understood. MiSeq amplicon high-throughput sequencing (Illumina®) is able to detect species richness (the number of species within a community) and species evenness (the sizes of species populations within a community). This study detected fungal diversity in discolored Mongolian pine for the first time by the MiSeq approach, focusing on the nuclear ribosomal internal transcribed spacer-1 (ITS1). The results show that the discolored wood was inhabited by a combination of microorganisms, more than 90% of which belong toAscomycotafungi at the phylum level. The MiSeq method revealed not only all the inhabited fungal species but also their quantitative relation to each other. The dominant fungal species in sample A areHelotiales(34.1%) andHypocreales(20.7%). The dominant fungal species in sample B isNectriaceae(67.9%), whileHypocrea(34.7%) andSporothrix(27.6%) are the dominant fungal species in sample C. It was confirmed via core microbiome analysis that the following fungi were common stain fungi in the three discolored samples:Fusariumspp.,Aspergillusspp.,Sporothrixspp.,Penicilliumspp.,Trichodermaspp.,Alternariaspp. andCladophialophoraspp.

Walking-induced vibration control in wood floors is a critical issue attracting the attention of many researchers and engineers. This paper presents an experimental study applying static deflection tests, modal tests, and pedestrian load tests to a series of full-scale 12 m span tooth plates connected to wood truss joist floors with strongbacks and partition walls. A comparison of the calculation error of vibration parameters between the theoretical formula and a numerical model was also conducted. The results show that strongbacks and partition walls effectively reduce both the vertical displacement and the root means acceleration at the center of the floor under pedestrian load but increases the natural frequency. The partition wall can achieve a better vibration-reduction effect than strongbacks. The error of the finite element model is higher than that of the theoretical formula. Using the theoretical formula in engineering wood floor design is recommended.