Background: Amputation is perceived differently across the globe hence the study was designed to determine the knowledge, attitude and acceptance of amputation among Amputee patients in the National Orthopeadic Hospital Enugu. Objectives: The objectives were to elicit the level of knowledge of amputation, determine the attitude of respondents towards amputation and determine level of acceptance of amputation. Materials and Methods: A descriptive cross sectional design was used and data was collected using questionnaire. The entire population (106) amputees were studied. Data was analyzed using (SPSS) version 24.0 and results presented in tables. Results: Findings revealed the mean age of the respondents 35+2.25years. All the respondents (100%) were aware that amputation is a treatment option to save live. The major factors that affects acceptance of amputation were preparation for the procedure (mean 3.26+ 2.37), fear of deformity (mean 3.15+ 2.68), and cost of prosthetics (mean 3.11+ 2.56), affordability of prosthetics (mean 3.57+ 3.07) availability of prosthetic device (mean 3.57+ 3.07) and public acceptance of amputees (mean 3.21+ 2.62).