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The objective of the study is to screen phytochemicals and develop chemical fingerprints of medicinally important Solanum indicum L. The powdered pant materials of leaf, fruit, stem and roots of S. indicum were extracted in methanol by soxhlet apparatus. Extracts were subjected to phytochemical screening and HPTLC fingerprints were developed. For development of fingerprints Cyclohexane: Ethyl acetate: Formic acid (6: 4: 1) was used as mobile phase. Phytochemical screening revealed the presence of alkaloids, cardiac glycosides, flavonoids, phenols, saponins and terpenoids in all the plant parts. Steroids were found present in leaves, fruits and roots whereas tannins were detected in leaves only. HPTLC fingerprinting of methanolic extracts of all plant parts has shown several peaks with different Rf values and peak areas. Phytoconstituents investigated have been described to have tremendous medicinal values in literature. HPTLC fingerprints would be helpful in identification, authentication and quality control of this species.

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