This investigation was carried out with the objective to study the effect of antioxidant semen additive ascorbic acid on cryopreservation of semen. Semen samples (n=10) from mature cattle bull stationed at Frozen semen laboratory, U.T. of Jammu & Kashmir, Hukkal, Jammu, were used to evaluate the effect of ascorbic acid additive at post-dilution and at post thaw stage. The semen sample was extended with Tris-Egg-Yolk-Citric-acid-Fructose-Glycerol (TEYCAFG) extender and were split into two groups: Group 1: TEYCAFG without any additive/ control and group 2: TEYCAFG + Ascorbic acid (5mM). Progressive motility, live spermatozoa, acrosomal integrity, sperm abnormality, hypo-osmotic swelling test (HOST) was evaluated at both post-dilution and post-thaw stage. Whereas, oxidative stress tests viz. malondialdehyde (MDA), catalase (CAT) and superoxide dismutase (SOD) were evaluated at only post-thawed stage. Group 2 i.e. ascorbic acid group, showed significant (p<0.05) increased live spermatozoa, acrosomal integrity and HOST positive spermatozoa, while significant (p<0.05) decreased sperm abnormalities in post-thawed semen. In oxidative stress evaluation the MDA level was significantly (p<0.05) decreased, whereas, SOD levels significantly (p<0.05) increased in group 2 in comparison to control group. It was concluded that addition of ascorbic acid (5 mM) as semen additive improves semen quality and minimize oxidative stress to the spermatozoa during cryopreservation of semen.

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