Background: Lohais a metal that is used in many preparations after transforming it into non-metallic form by purification and incineration method uses to treat different kinds of diseases. Mandura is the rusting of iron.Tryushanadyalauha(TL) is one among the Ayurvedicherbo-mineral formulations described in BhaishajyaRatnavalliand as modified dosage form as Tryushanadyamandura(TM).The herbal contents are tryushana (i.epippali( Piper longum Linn ),maricha (Piper nigrum Linn)and shunti (Zingiber Officinale Roscoe), cavya (Piper chabaHunter), bakuchi (Psoralea CorylifoliaLinn),bhang (cannabis sativum Linn),andlavana like saindhava,aubhida,vida and sauvarchala ,andlohabhasma is the main ingredient. Aim: Pharmaceutical and Analytical study of TryushanadyaLauha& modified form as TryushanadyaMandura and comparative evaluation for antidiabetic activity in Wistar rats. Materials and Methods: All herbal drugs will be collected, verified, and primarily authenticated by the Department of Dravyaguna. Lohaand Mandura will be procured from the vendor and authenticated by the Department of Rasashastra and BhaishajyaKalpana, Mahatma Gandhi Ayurved College Hospital Research Centre, Salod(H), Wardha, and they will be prepared as per reference. Organoleptic, bhasmapariksha, Physico-chemical,XRD and FEG-SEM parameters will be evaluated. To assess Tryushanadya Lauha (TL) and Tryushanadya Mandura (TM)antidiabetic action will be conducted in 30 Wistar rats in 5 groups and will be compared. Observation and Results: The study will be assessed Tryushanadya lauha (TL) and TryushanadyaMandura (TM)antidiabetic action in 30 Wistar rats by using one-way ANOVA. Conclusion: Pharmaceutical and Analytical study of TryushanadyaLauha (TL) & modified form as TryushanadyaMandura (TM) will provide the standard parameters.