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A novel method, BBD-SSPD is proposed by the combination of Box-Behnken Design (BBD) and Split-Split Plot Design (SSPD) which would ensure minimum number of experimental runs, leading to economical utilization in multi- factorial experiments. The brine shrimp Artemia was tested to study the combined effects of photoperiod, temperature and salinity, each with three levels, on the hatching percentage and hatching time of their cysts. The BBD was employed to select 13 treatment combinations out of the 27 possible combinations that were grouped in an SSPD arrangement. Multiple responses were optimized simultaneously using Derringer’s desirability function. Photoperiod and temperature as well as temperature-salinity interaction were found to significantly affect the hatching percentage of Artemia, while the hatching time was significantly influenced by photoperiod and temperature, and their interaction. The optimum conditions were 23 h photoperiod, 29 °C temperature and 28 ppt salinity resulting in 96.8% hatching in 18.94 h. In order to verify the results obtained from BBD-SSPD experiment, the experiment was repeated preserving the same set up. Results of verification experiment were found to be similar to experiment originally conducted. It is expected that this method would be suitable to optimize the hatching process of animal eggs.

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Grey mullet is a potential species for diversification of aquaculture due to fast growth, omnivorous feeding habits and the high market value of the fish as well as its value-added products such as salt-cured and dried eggs called 'bottarga' (Vinatea et al., 2018;Whitfield et al., 2012). The euryhaline and eurythermal characteristic of grey mullet helps in better adaptation to captive condition and makes it a suitable candidate for brackish water aquaculture.The nutritional attributes such as ω-3 and ω-6 fatty acids and essential amino acid contents make grey mullet as an appropriate