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There are several scientific works has been done with alternative medicines in preventive measurement of diabetes mellitus. The present study was designed to evaluated in-vitro antioxidant activity of newly developed three ayurvedic formulations of Alternanthera philoxeroides and quantifies possible groups of phyto-constituents present in it. The prepared ayurvedic formulations are 'swarasa kalpana', 'hima kalpana', and 'phanta kalpana', which were mentioned in the ayurvedic classical book 'Sarangadhar Samhita'. This is first effort to explore the potentially of these formulations by in-vitro antioxidant activity as well as level of antioxidant groups (total flavonoid, total flavonoid etc.). The Graph pad prism (Version-3) software evaluates the interrelationship between the formulations as well as the analyzed parameters. It can be concluded that the antioxidant activity might be responsible for the presence of phenolic, flavonoid and other phytoconstituents.