In the present study 1317 serum samples obtained from 6 broiler flocks, 2 broiler breeders and 28 commercial layer flocks were examined for HI antibodies of {NDV, IBV 4/91,, AIV (H5N1, H5N2, H5N3, H9, H7) and Adenov (EDS 76 )}. Studying the vaccination protocols in flocks under investigation and their seroconversion had led us to speculate and conclude viral affections in the different localities of Sharkia governorate. Speculating viral affections was a very hard task because the Egyptian market is jammed with a great variety of protective vaccines this was conflicting during result interpretation. Positive immune titers for AIV-H7 in sharkia governorate was detected at June /2014 in (El-salhia, 10 th of Ramadan, El-ibrahemia and Abo-hammed) in commercial layer flocks only. The seropositive samples that exceeded the cutoff values were 63 out of 1317 (4.8%). AIV-H9 high seropositive immune titers was constantly found in examined samples although their protective vaccines were neglected. AIV-H 5 N 3 seropositive results was recorded in a totally non vaccinated flocks against H 5 which reflect virus circulation in the poultry premises. Seropositive titer for IBV-D 274 and EDS 76 was recorded in a totally non vaccinated flocks against such antigens., which refer to their role in the total simultaneous incidence of disease and consequent mortality. From another point of view it should be noted that. Vaccinating chicken flocks following a ready made manuscript of the producing companies without prior evaluation of the maternally derived antibodies (MDA) or evaluating the immune titers before taking the decision of vaccination or even considering the disease situation in the area is possible cause for vaccine failure. Sentinel birds inclusion in poultry patches should be taken seriously to give a mirror for the circulating viral agents in the poultry premises. It worth to mention that a parallel bacteriological work was running during investigation of the causes of increased mortality or dropped egg production. This work revealed the isolation of a resistant bacteria of the (Kebseilla spp).