No abstract

The Field experiment was conducted at the research stationcollege of Agricultureuniversity of Baghdad / Al Jadria in 2016 -2017 using Randomized complete block design. The experiment consist of 12 treatments with three replicates , field experiment include the following factors , the first factor was biofertilization ) local and imported) , the second factor was the addation of 2 Level of chemical fertilizer (75% and 50%) of the recommended level , while the third factor include using of two type of carriers ( peatmoss or broth media ) , in addation to four control treatments (75% , 50% and 100 %) of recommended chemical fertilizer and control treatment of without bio or chemical fertilizer . Results showed the effect of the triple interaction of biofertilizer + mineral fertilizer + carriers . So the treatment local biofertilizer + 75% recommended mineral fertilizer + peat moss carrier gave the highest value for percentage of dry matter of vegatitive part 42.2% and also the same treatment was superior for plant height , percentage of tuber matter , mean tuber weight , tuber starch content , ‫واخرون‬ ‫راضي‬ ‫محليا‬ ‫المنتج‬ ‫الحيوي‬ ‫السماد‬ ‫تأثير‬35

The antimicrobial activity of Lactobacilli has been widely exploited for prevention offood –borne pathogens e.g.: Escherichia coli being the major cause of diarrhea especially inchildren, because of bacteriocin activity and the importance of herbal drugs, hence this studywas designed to evaluate the synergistic effect of plant extract and bacteriocin produced byLactobacillus on the growth and pathogenesis of Enteropathogenic Ecoli.1. The Plantaricin production was induced by adding the mutagenic agent Mitomycin C.2. Purification of Plantaricin was made by heating crude plantaricin at 80ºC for 10min andthen purified by two steps method including extraction with n-butanol followed by gelfiltration chromatography on Sepharose 6B column. The results showed that the specificactivity was 1600 AU/mg protein with 8 purification folds and 12% recovery yield.3. The antibacterial activity of Quercus infectoria with concentration 300 mg/ml wasshowed highly antibacterial activity in vitro and in vivo.4. The result showed synergistic effect of Plantaricin with Quercus infectoria extract afterexperimental infection that induced by orally dosing with Escherichia coli in vivo. A result ofhistopathological study was recorded recovery of tissue.

Isolation and diagnosis of bacillus mucilagionsus bacteria was done from different location of the rhrizospher soils. The diagnosis included studying the microscopical and biochemical properties for these isolates. The laboratory study included testing the isolates efficiency of isolates for solubilize potassium compounds in liquid and solid media (Modified Aleksandrov agar medium) and determination of its solubility coefficient. Results of isolation and diagnosis for 19 samples of rhizosphere soil planted with different crops (wheat , barley and vegetable crops)showed that only 7 isolates were dissolve potassium compounds with different degrees .The results of microscopical test showed these 7 isolates were rod-shape, gram positive, capsulated spore former and motile. On the other hand, the results of biochemical tests which include Catalase test, urease test, utilization of Glucose, Voges proskauer test, H 2 S production, utilization of ornithine, Nitrate reduces enzyme production, phenylalanine deaminase production, Lysine utilization, Gelatinase test and oxidase test. In addition to cultural properties showed that these isolates belong to bacillus mucilaginosus .Results showed that these isolates differ in their potassium solubility index with the range 1.22 to 2.25 while the sugar in the media effect on these solubility and glucose gave the superiority.