In current study use local ligninolytic crude extract enzyme (HAMU 8%) to improve IVDMD and reducing lignin content. The result suggest that treated wheat straw (WS), Corncobs (Cc), alfalfa hay (AH), by HAMU increased significantly (p <0.05) in vitro dry matter digestibility (IVDMD) and reduction in lignin content, However, extra enzyme improvement is shown with Corncobs treated with HAMU (average improvement, 19.84%) and lower improvement was shown in alfalfa hay treated with HAMU (average improvement, 10.07%). The higher reduction (P<0.01) in lignin content was shown in Cc treated with HAMU from 9.8 to 7.901%, with average reduction 1.899%; when the lower reduction was observed significantly (P<0.05) with AH treated from 8.26 to 7.77%, average reduction was 0.49%.It could be recommended, treated low quality roughages with HAMU improved the nutritional value of roughages through the digestion of Structural carbohydrates.
This study being performed at the central laboratory for graduate students Animal Resources Department / College of Agriculture / University of Baghdad/Aljadrea and in laboratory of applied microbiology Department, Food and Biotechnology Center /Directorate of Agriculture Research / Baghdad, Iraq. Wild strain of local ligninolytic bacteria of genus Streptomyces MS isolated from Iraq camel degrade feces was used to pre- treated of low quality roughages in in vitro fermentation. Roughages wheat straw (WS), corncobs (Cc), alfalfa hay (AH) was pre- treated with wild strain of genus Streptomyces MS. The results showed higher significant improvement (p<0.01) for in vitro dry matter digestibility (IVDMD %),in vitro Organic matter digestibility (IVOMD %) for most of roughages (WS Cc, AH). Moreover, greatest (p<0.01) improvement in(IVDMD,IVOMD %) was observed with pre-treated AH using Streptomyces MS prior of use for 21 days from 45 untreated to 67.07,67.08% treated, the smallest improving was observed (P<0.05) with WS from 20.78 to 30.22%. This result indicated that ability of Streptomyces MS to improve (IVDMD %) was significantly (P<0.05) affected by the roughages sources. Treated roughages with Streptomyces MS showed significant reduction in lignin content (p<0.01).However, the highest (p<0.01) reduction in lignin content was shown in Cc pre-treated with Streptomyces MS(from 9.8 to 6.87%), while lowest (P<0.05)reduction in lignin content was shown in AH (from8.26 to 7.64%), These results support the possibility application of this wild strain of bacteria to improve low quality roughages in ruminants feed.
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