Utilization of sugarcane bagasses for butanol, ethanol and acetone production was studied by acid hydrolysis and bacterial fermentation. Glucose, xylose and arabinose contents of sugarcane bagasse hydrolyzed in 5% (v/v) sulfuric acid solution were investigated in respective range of 5 to 60 min. Results showed glucose and xylose released during hydrolysis at 121 C and long treatment time of 60 minutes had high concentrations of 18.7 and 19.8 g/l, respectively. The sugarcane bagasse hydrolysate was then used for butanol, ethanol and acetone production by anaerobic fermentation using C.butyricum, C. sporogenes, C.beijerinckii and C.acetobutylicum. The maximum production based on solvent yield was 4.7 g/l butanol, 6.3 g/l ethanol and 9.7 g/l acetone obtained from fermentation of sugarcane bagasse hydrolysate using C. beijerinckii for 48 hours in the presence of 0.5% (w/v) sugarcane bagasse.