The aim of this study is to determine the emotional labor behavior of nurses who continue their education while working and its affecting factors. Material and Methods: The sample of the cross-sectional study consists of nursing graduate students who continue their education while working (n=173). Research data have been collected by "descriptive information form" and the HDEPS. Number, mean, percentage distribution, independent sample t-test/Mann-Whitney U test and one-way ANOVA/Kruskal-Wallis tests were used in the statistical analysis of the data. Results: The mean age of the nurses was 29.39±5.39, and 87.3% of them were women. Mean scores of the emotional labor behavior scale in nurses has been found like these; superficial behavior 3.87±0.50; in-depth behavior 3.94±0.46; and sincere behavior 4.04±0.57. The women's HDEPS in-depth behavior subscale average score was found to be significantly higher than that of men, while the HDEDS sincere behavior subscale score average of nurses working in private hospitals and receiving communication training was found to be significantly higher than the other groups. The HDEDS superficial behavior subscale mean score of the emergency service workers was significantly lower than the other groups; nurses working 40 hours or less per week had a significantly higher HDEPS superficial behavior subscale score than the other groups (p<0.05). Conclusion: It was concluded that the nurses showed the highest sincere behavior and the lowest superficial behavior, and that personal and professional factors were associated with emotional labor behavior.